山东农业大学学报(自然科学版)
山東農業大學學報(自然科學版)
산동농업대학학보(자연과학판)
JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2014年
4期
481-488,494
,共9页
黄艳艳%罗磊%牛庆霖%刘峰%翁曼丽%刘静%冯殿齐
黃豔豔%囉磊%牛慶霖%劉峰%翁曼麗%劉靜%馮殿齊
황염염%라뢰%우경림%류봉%옹만려%류정%풍전제
农杆菌介导法%香樟核糖体失活蛋白基因%枣疯病%Ca2+浓度
農桿菌介導法%香樟覈糖體失活蛋白基因%棘瘋病%Ca2+濃度
농간균개도법%향장핵당체실활단백기인%조풍병%Ca2+농도
Agrobacterium-mediated transformation%Cinnamomum camphora L. Ribosome-inactivating protein (RIP)%Jujube Witches Broom%Ca2+concentration
本研究应用农杆菌介导的方法,采用L9(34)正交试验设计,对影响宁阳大枣基因转化的各项因子进行了分析比较,并着重探索了Ca2+浓度对侵染转化的影响。结果表明:侵染效率最高的组合为1/2侵染液浓度,20 min侵染时间和0 mg/L Ca2+浓度。Ca2+浓度在提高宁阳大枣基因转化效率方面没有明显促进效果。Ca2+浓度仅对平均分化芽数影响较大,浓度为0 mg/L时,平均分化芽数最多。本试验共获得27株转化苗,试验转化效率为33.33%。
本研究應用農桿菌介導的方法,採用L9(34)正交試驗設計,對影響寧暘大棘基因轉化的各項因子進行瞭分析比較,併著重探索瞭Ca2+濃度對侵染轉化的影響。結果錶明:侵染效率最高的組閤為1/2侵染液濃度,20 min侵染時間和0 mg/L Ca2+濃度。Ca2+濃度在提高寧暘大棘基因轉化效率方麵沒有明顯促進效果。Ca2+濃度僅對平均分化芽數影響較大,濃度為0 mg/L時,平均分化芽數最多。本試驗共穫得27株轉化苗,試驗轉化效率為33.33%。
본연구응용농간균개도적방법,채용L9(34)정교시험설계,대영향저양대조기인전화적각항인자진행료분석비교,병착중탐색료Ca2+농도대침염전화적영향。결과표명:침염효솔최고적조합위1/2침염액농도,20 min침염시간화0 mg/L Ca2+농도。Ca2+농도재제고저양대조기인전화효솔방면몰유명현촉진효과。Ca2+농도부대평균분화아수영향교대,농도위0 mg/L시,평균분화아수최다。본시험공획득27주전화묘,시험전화효솔위33.33%。
In this study, the concentration effect of Ca2+on transformation of RIP gene (Ribosome Inactivating Protein Gene) cloned from Cinnamomum camphora to Ningyang Jujube was investigated in detail with L9(34) orthogonal design, through the analysis of factors which affected the transformation. The results showed that the concentration of Ca2+had no crucial effect on transformation and the best combination was the bacterium solution with 0.4-0.6 OD value was double diluted,20-minutes infection and without Ca2+. 27 transformation seedlings were obtained and the transformation rate was 33.33%.