CAJ | 학술논문

在实验室已获得的池蝶蚌（Hyriopsis schlegelii）部分细胞色素 P450基因序列的基础上，运用 RACE PCR的方法获得池蝶蚌细胞色素CYP基因（定义为 Hs-CYP450）的全长 cDNA。运用相关生物软件和在线软件对 Hs-CYP450基因序列以及其蛋白的一、二、三级结构进行了初步分析。结果显示，Hs-CYP450基因全长1809 bp， ORF框1431bp，共编码476个氨基酸；氨基酸组成成分中亮氨酸（Leu）含量最多，约9．5％，而最少的则是色氨酸，仅0．8％；通过疏水性分析显示，该蛋白属亲水性可溶性蛋白；二级结构预测显示该蛋白α螺旋（H）结构含量较多，约占46．22％，并均匀分布在蛋白中；β折叠（E）相对较少，约9．87％左右，多分布在两端。建立的系统进化树结果表明，Hs-CYP450基因序列与其他动物的细胞色素CYP基因有较高的同源性，在众多物种中和太平洋牡蛎（Cras-sostrea gigas）同源性相对较高。
재실험실이획득적지접방（Hyriopsis schlegelii）부분세포색소 P450기인서렬적기출상，운용 RACE PCR적방법획득지접방세포색소CYP기인（정의위 Hs-CYP450）적전장 cDNA。운용상관생물연건화재선연건대 Hs-CYP450기인서렬이급기단백적일、이、삼급결구진행료초보분석。결과현시，Hs-CYP450기인전장1809 bp， ORF광1431bp，공편마476개안기산；안기산조성성분중량안산（Leu）함량최다，약9．5％，이최소적칙시색안산，부0．8％；통과소수성분석현시，해단백속친수성가용성단백；이급결구예측현시해단백α라선（H）결구함량교다，약점46．22％，병균균분포재단백중；β절첩（E）상대교소，약9．87％좌우，다분포재량단。건립적계통진화수결과표명，Hs-CYP450기인서렬여기타동물적세포색소CYP기인유교고적동원성，재음다물충중화태평양모려（Cras-sostrea gigas）동원성상대교고。
In this study,Hs-CYP450 was cloned from by the method of RACE PCR.Then,relevant biologi-cal softwares and online softwares were used to make a preliminary analysis of Hs-CYP450 gene sequence, as well as the primary,secondary and tertiary structures of this protein.Our results indicated that the Hs-CYP450 cDNA present a full length of 1809bp that consisted of an open reading frame (ORF)of 1 431 bp encoding a protein of 476 amino acids,The content of leucine (Leu)accounted for 9.5% in the protein,but tryptophan content was only 0.8%;The hydrophobicity analysis showed that this protein was a hydrophilic soluble protein;The prediction of secondary structure suggested thatαhelix (H)content was 46.22% and distributed evenly in the protein;βfold (E)was about 9.87% and was located at both ends.The results of phylogenetic tree showed that Hs-CYP450 gene sequence had a high homology with other animals CYP gene,and the Crassostrea gigas shared the highest among these species.