南昌大学学报(理科版)
南昌大學學報(理科版)
남창대학학보(이과판)
JOURNAL OF NANCHANG UNIVERSITY(NATURAL SCIENCE)
2014年
4期
363-367
,共5页
冯蕾%马雅倩%谭婷%鄢爱平%郭岚%万益群
馮蕾%馬雅倩%譚婷%鄢愛平%郭嵐%萬益群
풍뢰%마아천%담정%언애평%곽람%만익군
高效液相色谱-串联质谱%酪氨酸%酪氨酸代谢产物
高效液相色譜-串聯質譜%酪氨痠%酪氨痠代謝產物
고효액상색보-천련질보%락안산%락안산대사산물
Tyrosine%Tyrosine metabolites%High-performance liquid chromatography with tandem mass spectrometry%Biological sample
建立了高效液相色谱-串联质谱法测定乳腺癌患者血清中多巴、酪氨酸、对羟苯基乳酸和酪胺的分析方法。在已优化的质谱条件下,待测物在6 min内完成分离,且效果良好。结果表明,4种物质的检出限在0.0005~0.20 mg·L-1之间;定量限在0.001~0.40 mg·L-1之间;线性相关系数在0.9989~0.9997之间,线性关系较好。血清中4种待测物在3个不同加标水平下的平均回收率在85.2%~114.0%之间,相对标准偏差在0.7%~8.5%之间。以上结果表明,该方法准确可靠,可用于实际样品的测定。
建立瞭高效液相色譜-串聯質譜法測定乳腺癌患者血清中多巴、酪氨痠、對羥苯基乳痠和酪胺的分析方法。在已優化的質譜條件下,待測物在6 min內完成分離,且效果良好。結果錶明,4種物質的檢齣限在0.0005~0.20 mg·L-1之間;定量限在0.001~0.40 mg·L-1之間;線性相關繫數在0.9989~0.9997之間,線性關繫較好。血清中4種待測物在3箇不同加標水平下的平均迴收率在85.2%~114.0%之間,相對標準偏差在0.7%~8.5%之間。以上結果錶明,該方法準確可靠,可用于實際樣品的測定。
건립료고효액상색보-천련질보법측정유선암환자혈청중다파、락안산、대간분기유산화락알적분석방법。재이우화적질보조건하,대측물재6 min내완성분리,차효과량호。결과표명,4충물질적검출한재0.0005~0.20 mg·L-1지간;정량한재0.001~0.40 mg·L-1지간;선성상관계수재0.9989~0.9997지간,선성관계교호。혈청중4충대측물재3개불동가표수평하적평균회수솔재85.2%~114.0%지간,상대표준편차재0.7%~8.5%지간。이상결과표명,해방법준학가고,가용우실제양품적측정。
A simple,selective,rapid and reliable method for the simultaneous determination of 3,4-di-hydroxyphenylalanine (DOPA),tyrosine (Tyr),4-hydroxyphenyllactic acid (PHPLA)and 4-hydroxyphen-ethylamine (TA)in human serum by high-performance liquid chromatography (HPLC)coupled with tan-dem mass spectrometry (MS/MS)was proposed for the first time.Under optimized conditions,all com-pounds were resolved within 6 min.The calibration curves were indicative of good linearity (correlation co-efficients >0.998 9),and the limits of detections and quantifications for Tyr and its metabolites ranged from 0.000 5 to 0.20 mg·L-1 and 0.001 to 0.40 mg·L-1 ,respectively.Recoveries of Tyr and its metabo-lites in breast cancer patient were in the range of 85.2%~114.0%,with RSDs of 0.7%~8.5%.The pres-ent method was successfully applied to the determination of DOPA,Tyr,PHPLA and TA in sera from breast cancer patients.