甘肃农业大学学报
甘肅農業大學學報
감숙농업대학학보
JOURNAL OF GANSU AGRICULTURAL UNIVERSITY
2014年
1期
105-110
,共6页
刺楸%遗传多样性%SRAP
刺楸%遺傳多樣性%SRAP
자추%유전다양성%SRAP
Kalopanax septemlobus%genetic diversity%SRAP
采用 SRAP标记对来自山东徂徕山、泰山、崂山、蒙山的71份刺楸种质进行遗传多样性分析.结果表明:12对引物共扩增出110条带,其中102条显示多态性,多态位点百分率(PPL)为92.73%;71份刺楸种质的遗传相似系数(GS)0.573~0.975,平均0.822,说明各种质间具有较高的遗传变异;种群间遗传分化系数(GST )为0.1360,表明有13.6%的变异存在于种群间,86.4%的变异存在于种群内,种群内的遗传分化显著大于种群间的遗传分化.UPGMA聚类表明71份种质在相似系数为0.71时可以聚为3类,总体上来源相同的种质优先聚类,但也存在交叉聚类.
採用 SRAP標記對來自山東徂徠山、泰山、嶗山、矇山的71份刺楸種質進行遺傳多樣性分析.結果錶明:12對引物共擴增齣110條帶,其中102條顯示多態性,多態位點百分率(PPL)為92.73%;71份刺楸種質的遺傳相似繫數(GS)0.573~0.975,平均0.822,說明各種質間具有較高的遺傳變異;種群間遺傳分化繫數(GST )為0.1360,錶明有13.6%的變異存在于種群間,86.4%的變異存在于種群內,種群內的遺傳分化顯著大于種群間的遺傳分化.UPGMA聚類錶明71份種質在相似繫數為0.71時可以聚為3類,總體上來源相同的種質優先聚類,但也存在交扠聚類.
채용 SRAP표기대래자산동조래산、태산、로산、몽산적71빈자추충질진행유전다양성분석.결과표명:12대인물공확증출110조대,기중102조현시다태성,다태위점백분솔(PPL)위92.73%;71빈자추충질적유전상사계수(GS)0.573~0.975,평균0.822,설명각충질간구유교고적유전변이;충군간유전분화계수(GST )위0.1360,표명유13.6%적변이존재우충군간,86.4%적변이존재우충군내,충군내적유전분화현저대우충군간적유전분화.UPGMA취류표명71빈충질재상사계수위0.71시가이취위3류,총체상래원상동적충질우선취류,단야존재교차취류.
The genetic diversity of Kalopanax septemlobus from Shandong was investigated by using SRAP marker.The results showed that 1 10 bands were amplified by 12 pairs of informative and reliable primers,of which 102 were polymorphic,resulting in a polymorphic rate of 92.73%.GS values of 71 K . septemlobus materials were 0.573~0.975,whose average was 0.822.There was higher genetic diversity a-mong all the materials.The genetic differentiation coefficient in populations was 0.136,there were vari-ances of 13.6% in populations,86.4% genetic variation existed within populations.This data illustrated genetic variation within population was larger than that among populations.UPGMA cluster analysis showed that 71 materials were classified into 3 groups when the similarity coefficient was 0.71.Overall, materials from the same source mostly clustered together,but cross clustering was also exist.Finally,we discussed the cause of the endangerment status K .septemlobus and proposed some management strategies for effectively protecting the species.