CAJ | 학술논문

糖苷生物碱(SGAs)是一类存在于茄科和百合科植物的重要次生代谢物,与植物的抗逆性和产品品质有密切关系。茄啶葡糖基转移酶(SGT2)是 SGAs合成代谢途径的末端关键酶之一,研究其编码基因的启动子序列对于 SGAs生物合成代谢调控有重要的作用和意义。本研究采用染色体步移技术,克隆到马铃薯茄啶葡糖基转移酶基因(sgt2)起始密码子上游2098 bp的启动子序列,已注册到GenBank(注册号：KC331038)。构建该启动子驱动报告基因gfp∶∶gus 的植物双元表达载体 p1304sgt2p,采用农杆菌介导的烟草叶片瞬时表达,通过 GUS组织化学染色分析 sgt2p启动子的活性。结果表明：gus 基因在转化烟草叶片中高效表达,克隆的启动子具有活性。
당감생물감(SGAs)시일류존재우가과화백합과식물적중요차생대사물,여식물적항역성화산품품질유밀절관계。가정포당기전이매(SGT2)시 SGAs합성대사도경적말단관건매지일,연구기편마기인적계동자서렬대우 SGAs생물합성대사조공유중요적작용화의의。본연구채용염색체보이기술,극륭도마령서가정포당기전이매기인(sgt2)기시밀마자상유2098 bp적계동자서렬,이주책도GenBank(주책호：KC331038)。구건해계동자구동보고기인gfp∶∶gus 적식물쌍원표체재체 p1304sgt2p,채용농간균개도적연초협편순시표체,통과 GUS조직화학염색분석 sgt2p계동자적활성。결과표명：gus 기인재전화연초협편중고효표체,극륭적계동자구유활성。
The potato steroidal glycoalkaloids(SGAs)is a kind of important secondary metabolite in So-lanace and Liliaceae.It is closely related to the stress resistance and product quality of plant.Solanidine glu-cosyltransferase is one of the terminases of SGAs synthetic metabolic pathway,to study the promoter of its coding gene has important significance in the regulation of the SGAs biosynthesis metabolism in plant.A 2 098 bp promoter sequence of 5′upstream of initial codon of sgt2 gene was obtained from Solanum tubero-sum by using the genome walking.The promoter sequence had been submitted to the GenBank(accession number:KC331038).The promoter sequence was fused with gfp∶∶gus gene originated from pCAM-BIA1304 to construct a binary vector p1304sgt2p,which was introduced into wild type tobacco leaves through Arobacterium-mediated transformation.The sgt2 gene promoter activity in sgt2p∶∶gfp∶∶gus transgenic tobacco leaves was determined through histochemical staining.The GUS staining showed that gus gene was highly expressed in transgenic tobacco leaves,the promoter cloned had activity .