甘肃农业大学学报
甘肅農業大學學報
감숙농업대학학보
JOURNAL OF GANSU AGRICULTURAL UNIVERSITY
2014年
1期
21-25
,共5页
母羊%雌二醇%孕酮%E2/P4
母羊%雌二醇%孕酮%E2/P4
모양%자이순%잉동%E2/P4
ewes%estrogen%progesterone%E2/P4
随机选取6只妊娠母羊,采集妊娠期各阶段的血样,分别用化学发光免疫分析法和放射免疫法测定血样中 E2和P4的浓度变化,探讨‘永昌甘农’肉羊新品种群母羊在妊娠期外周血液中雌二醇(E2)和孕酮(P4)浓度变化规律.结果显示：母羊妊娠期20 d之前外周血液中 E2、P4的浓度变化同发情周期相似,E2的浓度在第0天最高,为(133.678±60.892)pg/mL,第20~40天呈脉冲式平稳变化,从第45天开始上升,并在第90天出现一个峰值(80.757±42.503)pg/mL,之后缓慢下降.P4的浓度变化则是在第10天的峰值之后,即第15天出现一个下降的值(0.627±0.081)ng/mL,之后缓慢回升,分别在第75天和第90天出现2个峰值(3.211±2.375)ng/mL和(5.472±3.422)ng/mL.E2/P4的比值变化在妊娠期前20 d差异较大,20 d之后的数值变化平稳,相互之间差异不显著(P>0.05).因此,妊娠期第20~40天的 E2、P4浓度变化平稳,明显不同于正常发情周期的变化规律,可以作为母羊早期妊娠诊断的数据依据.
隨機選取6隻妊娠母羊,採集妊娠期各階段的血樣,分彆用化學髮光免疫分析法和放射免疫法測定血樣中 E2和P4的濃度變化,探討‘永昌甘農’肉羊新品種群母羊在妊娠期外週血液中雌二醇(E2)和孕酮(P4)濃度變化規律.結果顯示：母羊妊娠期20 d之前外週血液中 E2、P4的濃度變化同髮情週期相似,E2的濃度在第0天最高,為(133.678±60.892)pg/mL,第20~40天呈脈遲式平穩變化,從第45天開始上升,併在第90天齣現一箇峰值(80.757±42.503)pg/mL,之後緩慢下降.P4的濃度變化則是在第10天的峰值之後,即第15天齣現一箇下降的值(0.627±0.081)ng/mL,之後緩慢迴升,分彆在第75天和第90天齣現2箇峰值(3.211±2.375)ng/mL和(5.472±3.422)ng/mL.E2/P4的比值變化在妊娠期前20 d差異較大,20 d之後的數值變化平穩,相互之間差異不顯著(P>0.05).因此,妊娠期第20~40天的 E2、P4濃度變化平穩,明顯不同于正常髮情週期的變化規律,可以作為母羊早期妊娠診斷的數據依據.
수궤선취6지임신모양,채집임신기각계단적혈양,분별용화학발광면역분석법화방사면역법측정혈양중 E2화P4적농도변화,탐토‘영창감농’육양신품충군모양재임신기외주혈액중자이순(E2)화잉동(P4)농도변화규률.결과현시：모양임신기20 d지전외주혈액중 E2、P4적농도변화동발정주기상사,E2적농도재제0천최고,위(133.678±60.892)pg/mL,제20~40천정맥충식평은변화,종제45천개시상승,병재제90천출현일개봉치(80.757±42.503)pg/mL,지후완만하강.P4적농도변화칙시재제10천적봉치지후,즉제15천출현일개하강적치(0.627±0.081)ng/mL,지후완만회승,분별재제75천화제90천출현2개봉치(3.211±2.375)ng/mL화(5.472±3.422)ng/mL.E2/P4적비치변화재임신기전20 d차이교대,20 d지후적수치변화평은,상호지간차이불현저(P>0.05).인차,임신기제20~40천적 E2、P4농도변화평은,명현불동우정상발정주기적변화규률,가이작위모양조기임신진단적수거의거.
In order to explore the changes of E2 and P4 concentration in peripheral blood of meat sheep‘Yongchang Gannong’new breeding population pregnant ewes,6 ewes were selected random from ewes groups,taken blood sample in the stage of ewe gestation period,and E2 and P4 concentration were deter-mined by CLIA and RIA method respectively.The results showed that the changes of E2 and P4 concentra-tion in peripheral blood of gestation ewe was similar with the estrous cycle before 20 d of pregnant period, E2 concentrations reached the highest at 0th days.From 20th to 40th days,the concentration pulse changed smoothly,and from 45th day began to rising,the emergence of a peak at 90th days (80.757 ± 42.503 ) pg/mL then decreased slowly.The concentration of P4 declined in the value of (0.627±0.081)ng/mL at 15 th days after the fist peak at 10th days.After 20th rising slowly,appeared two peak at 75th and 90th days (3.21 1±2.375)ng/mL and (5.472 ± 3.422)ng/mL.E2/P4 ratio changed in the pregnancy early varies greatly,numerical variation steady after 20th days,the difference was not significant (P>0.05).It is sug-gested that the changes of E2 and P4 concentration of new breeding ewes pregnancy can be used as ewes pregnancy diagnosis data.
