分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2014年
1期
10-14
,共5页
邓绮文%丁宇%唐开宇%谭文城%苏箔金%郭禹标%杨惠玲
鄧綺文%丁宇%唐開宇%譚文城%囌箔金%郭禹標%楊惠玲
산기문%정우%당개우%담문성%소박금%곽우표%양혜령
鼻咽癌%14-3-3σ%反相蛋白质阵列%信号通路
鼻嚥癌%14-3-3σ%反相蛋白質陣列%信號通路
비인암%14-3-3σ%반상단백질진렬%신호통로
Nasopharyngeal carcinoma%14-3-3σ%RPPA%Signaling pathway
目的：探讨14-3-3σ对鼻咽癌CNE-2细胞的信号通路的影响。方法通过反相蛋白质阵列检测14-3-3σ转染鼻咽癌CNE-2细胞前后特定蛋白质表达量的变化。结果10种蛋白的表达量在14-3-3σ转染CNE-2细胞的前后发生了大于15%的变化，分别是Akt与pAkt（S473），mTOR与pmTOR，ERK2，p-p38，pSTAT3（Y705）与pSTAT3（S727）及Rb与p-Rb。结论14-3-3σ在鼻咽癌CNE-2细胞中所起的作用可涉及4条信号通路，分别是Akt通路、MAPK通路、JAK-STAT通路及Rb-E2f通路。
目的：探討14-3-3σ對鼻嚥癌CNE-2細胞的信號通路的影響。方法通過反相蛋白質陣列檢測14-3-3σ轉染鼻嚥癌CNE-2細胞前後特定蛋白質錶達量的變化。結果10種蛋白的錶達量在14-3-3σ轉染CNE-2細胞的前後髮生瞭大于15%的變化，分彆是Akt與pAkt（S473），mTOR與pmTOR，ERK2，p-p38，pSTAT3（Y705）與pSTAT3（S727）及Rb與p-Rb。結論14-3-3σ在鼻嚥癌CNE-2細胞中所起的作用可涉及4條信號通路，分彆是Akt通路、MAPK通路、JAK-STAT通路及Rb-E2f通路。
목적：탐토14-3-3σ대비인암CNE-2세포적신호통로적영향。방법통과반상단백질진렬검측14-3-3σ전염비인암CNE-2세포전후특정단백질표체량적변화。결과10충단백적표체량재14-3-3σ전염CNE-2세포적전후발생료대우15%적변화，분별시Akt여pAkt（S473），mTOR여pmTOR，ERK2，p-p38，pSTAT3（Y705）여pSTAT3（S727）급Rb여p-Rb。결론14-3-3σ재비인암CNE-2세포중소기적작용가섭급4조신호통로，분별시Akt통로、MAPK통로、JAK-STAT통로급Rb-E2f통로。
Objective The aim of the research is to clarify the impact of 14-3-3σ on signaling pathway of CNE-2 cell. Methods Reverse phase protein array (RPPA) is used to detect the changes of specific protein after 14-3-3σtransfected into CNE-2 cell. Results Ten kinds of proteins are found to have significant changes exceeding 15%, including Akt, pAkt (S473), mTOR, pmTOR, ERK2, p-p38, pSTAT3 (Y705), pSTAT3 (S727), Rb and p-Rb. Conclusion Four signaling pathway may be involved in the effect of 14-3-3σon CNE-2 cell, including Akt signaling pathway, MAPK signaling pathway, JAK-STAT signaling pathway and Rb-E2f signaling pathway.
