CAJ | 학술논문

目的：研究口服淫羊藿苷（Icariin， ICA）是否能提高大鼠的峰值骨密度和骨质量，起到预防骨质疏松症的作用。方法1月龄雌性健康SD大鼠随机分为两组，每组12只。服药组每日灌服25 mg/kg淫羊藿苷，对照组（CON）灌服给予等体积蒸馏水。每周监测大鼠体重。每月检测实验鼠的骨密度，3个月后处死所有大鼠。取血测血清骨钙素（OC）和抗酒石酸性磷酸酶5b（TRACP 5b）的含量。取双侧股骨和胫骨分别进行骨密度检测、μCT扫描和生物力学评价。剥离心、肝、胃、肾、肾上腺和子宫后称重，计算器官指数，并做常规病理学检测。结果两组大鼠的体重始终无显著性差异（P＞0.05）；各脏器的器官指数均无明显差异，病理学观察未见异常改变。第1、第2月的全身骨密度无明显差别（P＞0.05），但3个月后，ICA组显著高于CON组，股骨骨密度存在相同趋势（P＜0.05）；ICA组的血清OC水平升高，而TRACP 5b含量下降（P＜0.05）；μCT检测结果是：ICA组的骨体积百分率、骨小梁厚度和骨小梁数量均高于CON组，但骨小梁分离度和模型系数显著低于CON组（P＜0.05）；股骨最大载荷、弹性模量和屈服强度均是ICA组显著高于CON组（P＜0.05）。结论口服淫羊藿苷可通过抑制骨吸收而促进骨形成，提高大鼠的峰值骨密度和骨质量，对预防各种原因引起的骨质疏松及骨质疏松性骨折具有十分重要的意义。
목적：연구구복음양곽감（Icariin， ICA）시부능제고대서적봉치골밀도화골질량，기도예방골질소송증적작용。방법1월령자성건강SD대서수궤분위량조，매조12지。복약조매일관복25 mg/kg음양곽감，대조조（CON）관복급여등체적증류수。매주감측대서체중。매월검측실험서적골밀도，3개월후처사소유대서。취혈측혈청골개소（OC）화항주석산성린산매5b（TRACP 5b）적함량。취쌍측고골화경골분별진행골밀도검측、μCT소묘화생물역학평개。박리심、간、위、신、신상선화자궁후칭중，계산기관지수，병주상규병이학검측。결과량조대서적체중시종무현저성차이（P＞0.05）；각장기적기관지수균무명현차이，병이학관찰미견이상개변。제1、제2월적전신골밀도무명현차별（P＞0.05），단3개월후，ICA조현저고우CON조，고골골밀도존재상동추세（P＜0.05）；ICA조적혈청OC수평승고，이TRACP 5b함량하강（P＜0.05）；μCT검측결과시：ICA조적골체적백분솔、골소량후도화골소량수량균고우CON조，단골소량분리도화모형계수현저저우CON조（P＜0.05）；고골최대재하、탄성모량화굴복강도균시ICA조현저고우CON조（P＜0.05）。결론구복음양곽감가통과억제골흡수이촉진골형성，제고대서적봉치골밀도화골질량，대예방각충원인인기적골질소송급골질소송성골절구유십분중요적의의。
Objective To investigate the effect of the oral medication of icariin (ICA) on bone mineral density (BMD) and bone quality in rats.Methods Twenty-four 1-month-old female SD rats were randomly divided into 2 groups, and each group had 12 rats.Rats in the medication group were given an oral administration of 25mg/kg icariin, while rats in control group (CON)were given an administration of same volume of distilled water.The body weight of all the rats was monitored weekly.And BMD of all the rats was measured monthly.All the rats were sacrificed after 3-month treatment.Blood was collected and the serum levels of osteocalcin (OC) and anti-tartaric acid phosphatase 5b (TRACP 5b) were measured.The bilateral femurs and the tibia were also collected and BMD detection,μCT scanning, and biomechanical evaluation were performed.The heart, the liver, the stomach, the kidney, the adrenal, and the uterus were all collected and weighted.The organ index was calculated and the routine pathological detection was performed.Results No significant difference of the body weight of the rats in both groups was observed during 3 months (P>0.05).The organ index of all the organs showed no significant difference, and the pathological observation also showed no abnormal changes.BMD of the total bone in both groups at the 1st and the 2nd month showed no significant difference (P>0.05), but 3 months later, BMD in ICA group was significantly higher than that in CON group, and the same trend was also observed in the BMD of the femur (P<0.05).The serum level of OC in ICA group increased while the level of TRACP 5b decreased (P<0.05).The result ofμCT scanning revealed that the bone volume/tissue volume, the trabecular thickness, and the trabecular number in ICA group were all higher than that in CON group, but the trabecular separation and the coefficient of the model were significant lower than that in CON group (P<0.05).The maximum load, the elasticity modulus, and structural model index (SMI) of the femur in ICA group were significantly higher than that in CON group ( P <0.05).Conclusion Oral medication of icariin can promote bone formation by inhibiting bone resorption, improve peak BMD and bone quality, which has significant importance for the prevention of osteoporosis and osteoporotic fractures caused by different reasons.