中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
2期
115-119,124
,共6页
刘禄林%高超%王啸%赵国阳%何银锋%李光飞%俞晨%沈光思%张鹏%王爱东%徐又佳
劉祿林%高超%王嘯%趙國暘%何銀鋒%李光飛%俞晨%瀋光思%張鵬%王愛東%徐又佳
류록림%고초%왕소%조국양%하은봉%리광비%유신%침광사%장붕%왕애동%서우가
去势%雌激素%铁代谢%铁调素
去勢%雌激素%鐵代謝%鐵調素
거세%자격소%철대사%철조소
Ovariectomy%Estrogen%Iron metabolism%Hepcidin
目的：在机体，铁调素（Hepcidin）、膜转铁蛋白（FPN）、二价金属转运蛋白1（DMT1）、十二指肠色素b（DCYTB）是调节铁代谢的重要通道蛋白；本研究通过观察大鼠去势后铁代谢相关通道蛋白基因表达的变化，探讨雌激素与铁代谢的关系。方法雌性大鼠12只，随机分为2组：一组大鼠切除双侧卵巢（去势组），一组切除卵巢周脂肪（对照组），12 w后取标本检测观察；观察指标：①酶联免疫吸附法（Elisa）检测雌二醇浓度，②全自动生化分析仪检测大鼠血清铁，③电感耦合等离子体发射光谱仪原子吸收法检测鼠胫骨铁含量、肝组织铁含量，④半定量RT-PCR法检测肝脏Hepcidin、FPN的表达，以及十二指肠FPN、DMT1、DCYTB的mRNA的表达。结果去势组3项指标（雌二醇19.45±2.28 ng/L、血清铁36.36±9.34μmol/L、胫骨铁含量41.00±5.59μg/g）比对照组相应指标（雌二醇34.98±4.48 ng/L、血清铁54.73±11.08μmol/L、胫骨铁含量66.92±21.94μg/g）显著降低（P＜0.05）；去势组肝脏铁含量（750.20±94.08μg/g）比对照组（369.60±61.87μg/g）显著升高（P＜0.05）；RT-PCR结果显示：两组均有肝脏Hepcidin和FPN mRNA表达，表达水平两组比较存在显著差异；两组均有十二指肠FPN、DMT1和DCYTB mRNA表达，其中FPN、DCYTB mRNA表达水平两组比较有显著性差异，DMT1两组比较无明显变化。结论大鼠去势后可以使调控铁代谢的基因通道发生改变，影响铁的摄入和分布。
目的：在機體，鐵調素（Hepcidin）、膜轉鐵蛋白（FPN）、二價金屬轉運蛋白1（DMT1）、十二指腸色素b（DCYTB）是調節鐵代謝的重要通道蛋白；本研究通過觀察大鼠去勢後鐵代謝相關通道蛋白基因錶達的變化，探討雌激素與鐵代謝的關繫。方法雌性大鼠12隻，隨機分為2組：一組大鼠切除雙側卵巢（去勢組），一組切除卵巢週脂肪（對照組），12 w後取標本檢測觀察；觀察指標：①酶聯免疫吸附法（Elisa）檢測雌二醇濃度，②全自動生化分析儀檢測大鼠血清鐵，③電感耦閤等離子體髮射光譜儀原子吸收法檢測鼠脛骨鐵含量、肝組織鐵含量，④半定量RT-PCR法檢測肝髒Hepcidin、FPN的錶達，以及十二指腸FPN、DMT1、DCYTB的mRNA的錶達。結果去勢組3項指標（雌二醇19.45±2.28 ng/L、血清鐵36.36±9.34μmol/L、脛骨鐵含量41.00±5.59μg/g）比對照組相應指標（雌二醇34.98±4.48 ng/L、血清鐵54.73±11.08μmol/L、脛骨鐵含量66.92±21.94μg/g）顯著降低（P＜0.05）；去勢組肝髒鐵含量（750.20±94.08μg/g）比對照組（369.60±61.87μg/g）顯著升高（P＜0.05）；RT-PCR結果顯示：兩組均有肝髒Hepcidin和FPN mRNA錶達，錶達水平兩組比較存在顯著差異；兩組均有十二指腸FPN、DMT1和DCYTB mRNA錶達，其中FPN、DCYTB mRNA錶達水平兩組比較有顯著性差異，DMT1兩組比較無明顯變化。結論大鼠去勢後可以使調控鐵代謝的基因通道髮生改變，影響鐵的攝入和分佈。
목적：재궤체，철조소（Hepcidin）、막전철단백（FPN）、이개금속전운단백1（DMT1）、십이지장색소b（DCYTB）시조절철대사적중요통도단백；본연구통과관찰대서거세후철대사상관통도단백기인표체적변화，탐토자격소여철대사적관계。방법자성대서12지，수궤분위2조：일조대서절제쌍측란소（거세조），일조절제란소주지방（대조조），12 w후취표본검측관찰；관찰지표：①매련면역흡부법（Elisa）검측자이순농도，②전자동생화분석의검측대서혈청철，③전감우합등리자체발사광보의원자흡수법검측서경골철함량、간조직철함량，④반정량RT-PCR법검측간장Hepcidin、FPN적표체，이급십이지장FPN、DMT1、DCYTB적mRNA적표체。결과거세조3항지표（자이순19.45±2.28 ng/L、혈청철36.36±9.34μmol/L、경골철함량41.00±5.59μg/g）비대조조상응지표（자이순34.98±4.48 ng/L、혈청철54.73±11.08μmol/L、경골철함량66.92±21.94μg/g）현저강저（P＜0.05）；거세조간장철함량（750.20±94.08μg/g）비대조조（369.60±61.87μg/g）현저승고（P＜0.05）；RT-PCR결과현시：량조균유간장Hepcidin화FPN mRNA표체，표체수평량조비교존재현저차이；량조균유십이지장FPN、DMT1화DCYTB mRNA표체，기중FPN、DCYTB mRNA표체수평량조비교유현저성차이，DMT1량조비교무명현변화。결론대서거세후가이사조공철대사적기인통도발생개변，영향철적섭입화분포。
Objective Hepcidin, FPN, DMT1, and DCYTB are iron-regulating channel proteins in the body.This study was to explore the relationship between estrogen and iron metabolism by observing the changes of the gene expression of iron metabolism related channel protein in ovariectomized rats.Methods Twelve female SD rats were randomly divided into 2 groups:OVX group and Sham group.Rats in OVX group underwent bilateral ovariectomy, while rats in Sham group were excised some fat tissue around the ovaries.Twelve weeks later, the specimen were collected.The serum concentration of E2 was detected using ELISA method. The serum iron was detected using were automatic biochemical analyzer.The iron content in the liver and the tibia was detected using an ICP tester.The expression of hepcidin and ferropotin (FPN) mRNA in the liver and the expression of FPN, DMT-1, and DCYTB mRNA in the duodenum were detected using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results The serum levels of E2 and iron, as well as the iron content in the tibia in OVX group were 19.45 ±2.28ng/L, 36.36 ± 9.34 μmol/L, and 41.00 ±5.59 μg/g, respectively, which were significantly lower than those in Sham group (34.98 ±4.48 ng/L, 54.73 ±11.08 μmol/L, and 66.92 ±21.94 μg/g, respectively, P<0.05).On the contrary, the iron content in the liver in OVX group was significantly higher than that in Sham group (750.20 ±94.08 μg/g VS 369.60 ±61.87 μg/g, P<0.05).The results of RT-PCR revealed that the expression of hepcidin and FPN mRNA in liver in both groups was observed, showing significant different between 2 groups.The expression of FPN, DMT-1, and DCYTB mRNA in the duodenum in both groups was observed, and the expression of FPN and DCYTB mRNA showed significant difference between 2 groups, while no significant change of the expression of DMT1 mRNA was observed.Conclusion The ovariectomy in rats may change the channel proteins modulating the iron metabolism, thus affecting the absorption and distribution of iron.
