CAJ | 학술논문

利用发光二极管(LED)作为光源，以冷百荧光灯光作为对照，研究不同光质[红光637 nm、绿光529 nm、蓝光453 nm、白光(400-700) nm]对念珠藻葛仙米生长和生理生化特性的影响。结果表明：在培养前期，红光促进藻蓝蛋白合成，而藻红蛋白合成受抑制；蓝光和绿光则促进藻蓝蛋白合成。在培养后期，红光处理有利于叶绿素 a和类胡萝卜素积累，其含量分别达到干重的1.33%和0.24%；绿光、白光和冷白荧光培养物的相应色素的含量均约占1.0%和0.16%；蓝光培养物的相应色素含量分别仅为0.45%和0.11%。红光培养物的氨基酸含量达干重的23.1%，是对照的1.58倍。除蓝光外其他光质对还原糖的含量影响无显著差异。在培养过程中LED白光和冷白荧光培养物的平均相对生长速率分别约为其他色光培养物的1.3和1.5倍。
이용발광이겁관(LED)작위광원，이랭백형광등광작위대조，연구불동광질[홍광637 nm、록광529 nm、람광453 nm、백광(400-700) nm]대념주조갈선미생장화생리생화특성적영향。결과표명：재배양전기，홍광촉진조람단백합성，이조홍단백합성수억제；람광화록광칙촉진조람단백합성。재배양후기，홍광처리유리우협록소 a화류호라복소적루，기함량분별체도간중적1.33%화0.24%；록광、백광화랭백형광배양물적상응색소적함량균약점1.0%화0.16%；람광배양물적상응색소함량분별부위0.45%화0.11%。홍광배양물적안기산함량체간중적23.1%，시대조적1.58배。제람광외기타광질대환원당적함량영향무현저차이。재배양과정중LED백광화랭백형광배양물적평균상대생장속솔분별약위기타색광배양물적1.3화1.5배。
The influence of different Light-Emitting Diode (LED) wavelengths (red 637 nm, green 529 nm, blue 453 nm and white light, set cool white fluorescence as control) on the growth and the physiological and biochemical characte-ristics of Nostoc sphaeroides Kützing were studied. Results showed that in the initial growth stage, red light promoted the synthesis of phycocyanin and inhibited the phycoerythrin, and the former was also increased by blue and green lights. In the late growth stage, the contents of chlorophyll a and carotenoids in the colonies which cultured under red light accounted for 1.33%and 0.24%of the dry weight, while they were about 1.0%and 0.16%respectively in green, white, or cool white fluorescence cultures, and 0.45%and 0.11%respectively in blue light culture. The content of total amino acids in red light culture was 23.1%in dry weight, which was 1.58 times of the control group. There was no significant difference between the contents of reducing sugars in the cultures under different light wavelengths except blue light. The mean specific growth rates of the white light culture and the cool white fluorescence cultures were about 1.3 and 1.5 times of those in other monochromatic light cultures, respectively.