CAJ | 학술논문

β-环柠檬醛(β-cyclocitral)是由微囊藻产生的主要藻源性异味污染物之一。利用稀释涂平板法,从采集到的微囊藻水华水样中分离得到两株降解β-环柠檬醛的菌株DH16和DH18,16S rRNA序列比对和系统进化树分析表明它们分别属于食酸菌属(Acidovorax)和不动杆菌属(Acinetobacter)。实验室保藏的微囊藻毒素降解菌Novosphingobium sp. THN1对β-环柠檬醛也有降解效果,该菌属于新鞘氨醇杆菌属(Novosphingobium)。对三株菌进行以β-环柠檬醛为唯一碳源的培养实验,气相色谱检测分析表明DH18和THN1两株菌具有高效降解β-环柠檬醛的能力,可以作为研究β-环柠檬醛生物降解机理的理想材料。
β-배저몽철(β-cyclocitral)시유미낭조산생적주요조원성이미오염물지일。이용희석도평판법,종채집도적미낭조수화수양중분리득도량주강해β-배저몽철적균주DH16화DH18,16S rRNA서렬비대화계통진화수분석표명타문분별속우식산균속(Acidovorax)화불동간균속(Acinetobacter)。실험실보장적미낭조독소강해균Novosphingobium sp. THN1대β-배저몽철야유강해효과,해균속우신초안순간균속(Novosphingobium)。대삼주균진행이β-배저몽철위유일탄원적배양실험,기상색보검측분석표명DH18화THN1량주균구유고효강해β-배저몽철적능력,가이작위연구β-배저몽철생물강해궤리적이상재료。
β-cyclocitral, one of the most common taste and odour compounds, is mostly produced by water bloom forming Microcystis. Using the agar-plate method, bacterial strains DH16 and DH18 for degrading β-cyclocitral were isolated from the water samples in the Microcystis dominated water blooms. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that these strains belonged to genera Acidovorax and Acinetobacter respectively. The strain Novosphingobiums sp.THN1, a reported microcystin degraded strain, was also shown to have the ability to de-grade β-cyclocitral. The experiment using β-cyclocitral as the sole carbon source demonstrated that strains DH18 and THN1were able to decomposeβ-cyclocitral efficiently, and these bacterial strains could provide good model organisms for the studying the biodegradation ofβ-cyclocitral.