CAJ | 학술논문

目的：对人粪便中匹诺塞林浓度的高效液相色谱质谱联用（LC-MS/MS）定量分析方法的测量不确定度进行评定。方法：分析人粪便中匹诺塞林浓度测定过程，确定并简化不确定度来源，利用LC-MS/MS测定人粪便中匹诺塞林的方法学验证数据，通过统计学方法，依次计算各变量的不确定度、合成不确定度和扩展不确定度。结果：人粪便中低（51.07 ng·mL-1）、中（510.44 ng·mL-1）和高（2957.50 ng·mL-1）质量浓度匹诺塞林的扩展不确定度分别为14.58 ng·mL-1、26.30 ng·mL-1和206.46 ng·mL-1（k=2，置信概率为95%）。结论：测量不确定度与检测结果数值相关，随浓度高低不同而有所差异，而且测定不确定度比例在匹诺塞林不同浓度的结果也不相同，标准曲线拟合对低浓度点的影响非常大。
목적：대인분편중필낙새림농도적고효액상색보질보련용（LC-MS/MS）정량분석방법적측량불학정도진행평정。방법：분석인분편중필낙새림농도측정과정，학정병간화불학정도래원，이용LC-MS/MS측정인분편중필낙새림적방법학험증수거，통과통계학방법，의차계산각변량적불학정도、합성불학정도화확전불학정도。결과：인분편중저（51.07 ng·mL-1）、중（510.44 ng·mL-1）화고（2957.50 ng·mL-1）질량농도필낙새림적확전불학정도분별위14.58 ng·mL-1、26.30 ng·mL-1화206.46 ng·mL-1（k=2，치신개솔위95%）。결론：측량불학정도여검측결과수치상관，수농도고저불동이유소차이，이차측정불학정도비례재필낙새림불동농도적결과야불상동，표준곡선의합대저농도점적영향비상대。
Objective: To set out the procedures for evaluation of measurement uncertainty for the determination of pinocembrin in human feces by high-performance liquid chromatography-tandem mass spectrometry. Methods: Detection process was analyzed. Uncertainty sources were established and simplified, then calculated each component of uncertainty, combined uncertainty and expanded uncertainty. Results: The expand uncertainty of pinocembrin with low (51.07 ng·mL-1), medium (510.44 ng·mL-1) and high)2957.50 ng·mL-1)concentration was 14.58 ng·mL-1, 26.30 ng·mL-1 and 206.46 ng·mL-1, respectively (k=2,conifdence probability was 95%). Conclusions: Uncertainty for the determination of pinocembrin in human feces was related to the detecting data, and the proportion of uncertainty was different in low, medium and high concentration. The standard curve iftting had a great inlfuence on the uncertainty at the low level of pinocembrin.