CAJ | 학술논문

提出了高效液相色谱法测定蜜饯中人工合成甜味剂对乙氧基苯脲含量的方法。样品采用醋酸-醋酸铵缓冲液超声提取,全自动固相萃取仪净化,SB-C18反相色谱柱分离,荧光检测器检测。对乙氧基苯脲在0~10 mg/L范围内的线性相关系数为0.9987,方法定量限(S/N=10)小于0.1 mg/kg。以三种空白蜜饯为基体,在3个添加水平进行加标回收试验,平均回收率在81.7%~92.4%之间,相对标准偏差(n=6)在2.4%~6.8%之间。
제출료고효액상색보법측정밀전중인공합성첨미제대을양기분뇨함량적방법。양품채용작산-작산안완충액초성제취,전자동고상췌취의정화,SB-C18반상색보주분리,형광검측기검측。대을양기분뇨재0~10 mg/L범위내적선성상관계수위0.9987,방법정량한(S/N=10)소우0.1 mg/kg。이삼충공백밀전위기체,재3개첨가수평진행가표회수시험,평균회수솔재81.7%~92.4%지간,상대표준편차(n=6)재2.4%~6.8%지간。
High performance liquid chromatography is applied for the determination of artificial sweete-ner 4-Ethoxyphenylurea in succade.The sample is ultrasonic extracted with acetic acid/ammonium acetate buffer solution and purified by automatic solid-phase extraction.The extract is separated by SB-C1 8 column and detected by fluorescence detector.The value of correlation coefficient in the range of 0 to 10 mg/L is 0.9987.The limit of quantity (S/N=10)is less than 0.1 mg/kg.Using blank sample of succade as matrixes,the recovery is tested at 3 different concentration levels and the values of recovery are in the range of 81.7% to 92.4% with RSDs (n=6)in the range of 2.4% to 6.8%.