CAJ | 학술논문

目的：观察2型糖尿病小鼠的骨代谢及骨微结构的特点。方法采用雄性KK/Upj-Ay/J小鼠（自发性2型糖尿病模型小鼠）10只作为实验组，同时选用10只雄性C57BL/6小鼠作为对照组。两组小鼠均给予常规饲料喂养，确认KK/Upj-Ay/J小鼠发病，继续饲养12周后处死所有小鼠，测定血清骨碱性磷酸酶（ BALP）及抗酒石酸酸性磷酸酶（ TRAP）活性，并运用Micro-CT分析小鼠胫骨微结构定量参数。结果与对照组相比，2型糖尿病小鼠血清BALP活性明显下降（分别为对照组：0.029±0.003μU/min，T2DM组：0.014±0.003μU/min，P＜0.05），血清TRAP活性明显升高（分别为对照组：0.513±0.034 U/L， T2DM组：0.701±0.054 U/L，P＜0.05），胫骨平台处骨密度明显下降（对照组：810.000±21.000 mg/cm3，T2DM组：709.000±18.000 mg/cm3，P＜0.05）。结论2型糖尿病小鼠的骨吸收加快而骨形成不足，导致其骨量下降及骨折风险增大。
목적：관찰2형당뇨병소서적골대사급골미결구적특점。방법채용웅성KK/Upj-Ay/J소서（자발성2형당뇨병모형소서）10지작위실험조，동시선용10지웅성C57BL/6소서작위대조조。량조소서균급여상규사료위양，학인KK/Upj-Ay/J소서발병，계속사양12주후처사소유소서，측정혈청골감성린산매（ BALP）급항주석산산성린산매（ TRAP）활성，병운용Micro-CT분석소서경골미결구정량삼수。결과여대조조상비，2형당뇨병소서혈청BALP활성명현하강（분별위대조조：0.029±0.003μU/min，T2DM조：0.014±0.003μU/min，P＜0.05），혈청TRAP활성명현승고（분별위대조조：0.513±0.034 U/L， T2DM조：0.701±0.054 U/L，P＜0.05），경골평태처골밀도명현하강（대조조：810.000±21.000 mg/cm3，T2DM조：709.000±18.000 mg/cm3，P＜0.05）。결론2형당뇨병소서적골흡수가쾌이골형성불족，도치기골량하강급골절풍험증대。
Objective To observe the effect of type 2 diabetes mellitus (T2DM) on bone metabolism and bone microstructure in mice.Methods Ten male KK/Upj-Ay/J mice, a mouse model of T2DM, were selected as experimental group, and 10 male C57BL/6 mice were selected as control group.The mice in both groups were fed with routine fodder.After the confirmation of T2DM in KK/Upj-Ay/J mice, all mice were fed for another 12 weeks and then executed.The activity of serum bone alkaline phosphatase (BALP) and tartrate-resistant acid phosphatase ( TRAP) were detected.The parameters of the tibia were analyzed using micro-CT.Results Compared with those in control group, the activity of serum BALP in T2DM mice decreased significantly (control group:0.029 ±0.003 μU/min; T2DM group: 0.014 ±0.003 μU/min, P<0.05), while the activity of serum TRAP increased significantly (control group:0.513 ±0.034 U/L;T2DM group:0.701 ±0.054 U/L, P<0.05).The bone mineral density of the tibial plateau decreased significantly in T2DM group compared with that in control group ( control group:810.000 ±21.000 mg/cm3; T2DM group:709.000 ±18.000 mg/cm3, P<0.05).Conclusion The bone resorption in mice with T2DM accelerates while the bone formation is deficient, resulting in osteopenia and increased risk of bone fracture.