CAJ | 학술논문

目的：建立桑枝药材中黄酮类物质高效液指纹图谱（HPLC-DAD）的分析方法。方法采用 HPLC-DAD 法对桑枝进行指纹图谱分析，以紫云英苷为参照物分析10批不同产地的桑枝样品，采用计算机辅助相似性评价系统进行评价。结果桑枝药材 HPLC 指纹图谱由11个特征峰构成，相似度均＞0．90，符合指纹图谱研究技术的要求。结论所建立的指纹图谱结果稳定，重现性好，该方法准确、简单，其指纹图谱可用于桑枝药材的鉴别和质量控制。
목적：건립상지약재중황동류물질고효액지문도보（HPLC-DAD）적분석방법。방법채용 HPLC-DAD 법대상지진행지문도보분석，이자운영감위삼조물분석10비불동산지적상지양품，채용계산궤보조상사성평개계통진행평개。결과상지약재 HPLC 지문도보유11개특정봉구성，상사도균＞0．90，부합지문도보연구기술적요구。결론소건립적지문도보결과은정，중현성호，해방법준학、간단，기지문도보가용우상지약재적감별화질량공제。
Objective To establish an analytical method of HPLC fingerprint of flavonoids ingredients in Ramulus Mori .Methods The HPLC-DAD method was used to analyze the fingerprint ofRamulus Mori . 10 batches of samples of different origin were analyzed with astragalin served as the reference.Computer-aided evaluation system of similarity was used to evaluate.Results The fingerprints constituted by the 11 characteristic peak,the similarity of fingerprints of 10 batches of samples was greater than 0.9,which met the requirements of fingerprints technology.Conclusion The established fingerprints had good stability and reproducibility,which could be used to identify and control the quality of Ramulus Mori .