CAJ | 학술논문

目的：培养哈萨克族（哈族）成人食管正常上皮细胞，建立能够在体外长期培养的哈族食管上皮细胞系，为上皮细胞的体外研究提供实验材料。方法取哈萨克族食管癌患者正常食管上皮，用0．25％中性蛋白酶（DispaseⅡ）和0．05％胰蛋白酶/0．03％ EDTA 联合消化获取哈族食管上皮细胞，使用 EpiCM-2无血清培养基培养，通过细胞形态学和角蛋白免疫组织化学法鉴定培养的食管正常上皮细胞的来源。结果首次培养的原代哈萨克族正常食管上皮细胞经8～10 d 后可融合成片，融合率为80％～90％，细胞呈“铺路石”样生长，细胞角蛋白表达阳性，可连续传代，传代的细胞经3～5 d 可达到80％～90％融合。结论建立的体外分离培养哈族成人食管正常上皮细胞的方法方便可行。
목적：배양합살극족（합족）성인식관정상상피세포，건립능구재체외장기배양적합족식관상피세포계，위상피세포적체외연구제공실험재료。방법취합살극족식관암환자정상식관상피，용0．25％중성단백매（DispaseⅡ）화0．05％이단백매/0．03％ EDTA 연합소화획취합족식관상피세포，사용 EpiCM-2무혈청배양기배양，통과세포형태학화각단백면역조직화학법감정배양적식관정상상피세포적래원。결과수차배양적원대합살극족정상식관상피세포경8～10 d 후가융합성편，융합솔위80％～90％，세포정“포로석”양생장，세포각단백표체양성，가련속전대，전대적세포경3～5 d 가체도80％～90％융합。결론건립적체외분리배양합족성인식관정상상피세포적방법방편가행。
Objective To culture normal Kazakh adult esophageal epithelial cells and establish a normal Ka-zakh esophageal epithelial cell line to provide experimental materials for further in-vitro studies.Methods Normal Kazakh esophageal epithelium samples were obtained from the normal part of the esophagus of a patient with esophageal carcinoma.The mucosal layer was dissociated into a single cell suspension by 0.25% Dispase Ⅱ and 0.05% trypsin/0.03% EDTA.Cells were grown in EpicM-2 Serum-free media.The cultured cells were identified through their morphological characteristics and immunohistochemical staining.Result The cultured cells showed microscopic features of epithelial cells and were positive in ker-atin and epithelial membrane antigen staining.About 8-10 days after primary culture,the cells displayed a cobblestone morphology and were passaged successfully.Conclusion The culture methods and techniques used in the experiments are convenient and suitable for the primary culture of Kazakh normal e-sophageal epithelial cells.