天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
3期
238-240
,共3页
曹国龙%刘小坡%冯云波%田发明
曹國龍%劉小坡%馮雲波%田髮明
조국룡%류소파%풍운파%전발명
骨质疏松%骨折%洛伐他汀%降钙素%骨密度%疾病模型,动物
骨質疏鬆%骨摺%洛伐他汀%降鈣素%骨密度%疾病模型,動物
골질소송%골절%락벌타정%강개소%골밀도%질병모형,동물
osteoporosis%fractures,bone%lovastatin%calcitonin%bone density%disease models,animal
目的：探讨洛伐他汀单独或联合降钙素对骨质疏松大鼠骨折愈合的影响。方法4月龄雌性SD大鼠40只，随机分为正常骨折（A）组、骨质疏松性骨折（B）组、洛伐他汀治疗（C）组、降钙素治疗（D）组和联合治疗（E）组，每组8只。A组行假手术，B、C、D、E组行双侧卵巢摘除术，术后8周制作股骨干骨折模型，骨折后8周处死大鼠。采用ELISA法检测血Ⅰ型前胶原氨基端前肽（PINP），X线摄片及评分观察骨折愈合情况，骨密度检测骨折侧股骨骨密度，生物力学分析骨折侧股骨最大载荷。结果（1）血清PINP水平：A组显著低于其余各组，C、E组显著高于B组，D组显著低于C组。（2）X线示A、E组愈合良好，B组多数标本可见模糊骨折线，C、D组略好于B组。评分结果：A、E组显著高于B、C、D组。（3）骨密度：股骨全长及中段骨密度A组最高，E、D、C组次之，B组最小。（4）最大载荷：A组大鼠骨折侧股骨最大载荷显著高于其余4组，E组显著高于B组。结论骨质疏松大鼠骨量下降、骨折愈合进程延迟，洛伐他汀联合降钙素较单独应用可更有效地抑制骨质疏松大鼠骨量的丢失并促进骨折的愈合。
目的：探討洛伐他汀單獨或聯閤降鈣素對骨質疏鬆大鼠骨摺愈閤的影響。方法4月齡雌性SD大鼠40隻，隨機分為正常骨摺（A）組、骨質疏鬆性骨摺（B）組、洛伐他汀治療（C）組、降鈣素治療（D）組和聯閤治療（E）組，每組8隻。A組行假手術，B、C、D、E組行雙側卵巢摘除術，術後8週製作股骨榦骨摺模型，骨摺後8週處死大鼠。採用ELISA法檢測血Ⅰ型前膠原氨基耑前肽（PINP），X線攝片及評分觀察骨摺愈閤情況，骨密度檢測骨摺側股骨骨密度，生物力學分析骨摺側股骨最大載荷。結果（1）血清PINP水平：A組顯著低于其餘各組，C、E組顯著高于B組，D組顯著低于C組。（2）X線示A、E組愈閤良好，B組多數標本可見模糊骨摺線，C、D組略好于B組。評分結果：A、E組顯著高于B、C、D組。（3）骨密度：股骨全長及中段骨密度A組最高，E、D、C組次之，B組最小。（4）最大載荷：A組大鼠骨摺側股骨最大載荷顯著高于其餘4組，E組顯著高于B組。結論骨質疏鬆大鼠骨量下降、骨摺愈閤進程延遲，洛伐他汀聯閤降鈣素較單獨應用可更有效地抑製骨質疏鬆大鼠骨量的丟失併促進骨摺的愈閤。
목적：탐토락벌타정단독혹연합강개소대골질소송대서골절유합적영향。방법4월령자성SD대서40지，수궤분위정상골절（A）조、골질소송성골절（B）조、락벌타정치료（C）조、강개소치료（D）조화연합치료（E）조，매조8지。A조행가수술，B、C、D、E조행쌍측란소적제술，술후8주제작고골간골절모형，골절후8주처사대서。채용ELISA법검측혈Ⅰ형전효원안기단전태（PINP），X선섭편급평분관찰골절유합정황，골밀도검측골절측고골골밀도，생물역학분석골절측고골최대재하。결과（1）혈청PINP수평：A조현저저우기여각조，C、E조현저고우B조，D조현저저우C조。（2）X선시A、E조유합량호，B조다수표본가견모호골절선，C、D조략호우B조。평분결과：A、E조현저고우B、C、D조。（3）골밀도：고골전장급중단골밀도A조최고，E、D、C조차지，B조최소。（4）최대재하：A조대서골절측고골최대재하현저고우기여4조，E조현저고우B조。결론골질소송대서골량하강、골절유합진정연지，락벌타정연합강개소교단독응용가경유효지억제골질소송대서골량적주실병촉진골절적유합。
Objective To investigate the effects of lovastatin alone or combined with calcitonin on fracture repair in osteoporotic rats. Methods Forty 4-month-old female SD rats were randomized into 5 groups(8 rats in each group):normal fractured group (A), osteoporotic fractured group (B), lovastatin treatment group(C), calcitonin treatment group (D) and lovastatin combined with calcitonin treatment group. All rats except group A received bilateral ovariectomy. The midshaft femur fracture model was established in all rats 8 weeks after operation. The serum level of procollagen amino-terminal propeptide (PINP) was assessed by ELISA. X-ray and bone mineral density detection was used to observe the fracture healing process. The maximal loading of femoral fractures was analyzed by biomechanical method. Results (1) The serum level of PINP was significantly lower in group A than that of other groups. There was a significantly higher level of PINP in group C and group E than that of group B, and the level of PINP was significantly lower in group D than that of group C. (2) The X-ray showed more progressed fracture healing in group A and group E. The accordingly score indicated that there was a markedly higher score in groups A and group E compared to that of other three groups. (3) There was a highest bone mineral density in the full-length and in the middle of femur bone in group A, followed by group E, group D and group C. The lowest bone mineral density was found in group B. (4) The biomechanical test showed that the maximal loading in femur fracture side was significantly higher in group A than that of other four groups, in which it was higher in group E than that of group B. Conclusion The osteoporosis decreased bone mass and delayed fracture healing process in rat model. The treatment of lovastatin combined with calcitonin showed more positive effect on preventing bone loss and promoting fracture repair than lovastatin alone.
