CAJ | 학술논문

目的：观察鞣花酸对黑素细胞（melanocyte, MC）合成黑素以及传递黑素的影响并探讨其机制。方法分别纯化培养来自人包皮的表皮黑素细胞和角质形成细胞(keratinocyte, KC)，传第2代后以1∶10的比例将两细胞接种到3 cm×3 cm的小培养皿中，单独或混合培养的细胞经高、中、低（100、10、1 mg/L）3种浓度的鞣花酸干预48 h后，分别检测干预前后黑素细胞中黑素含量、酪氨酸酶活性的变化，采用流式法检测混合培养细胞中黑素传递的情况，以10 nmol/L熊果苷为阳性对照。结果3种浓度鞣花酸浓度均可下调细胞酪氨酸酶活性，并呈浓度依赖性。除了1 mg/L的鞣花酸对黑素细胞黑素合成无明显作用，其余浓度的鞣花酸均可降低黑素含量。鞣花酸同时具有抑制黑素传递的作用。结论鞣花酸可抑制黑素合成及黑素传递，或可作为一种皮肤美白剂应用。
목적：관찰유화산대흑소세포（melanocyte, MC）합성흑소이급전체흑소적영향병탐토기궤제。방법분별순화배양래자인포피적표피흑소세포화각질형성세포(keratinocyte, KC)，전제2대후이1∶10적비례장량세포접충도3 cm×3 cm적소배양명중，단독혹혼합배양적세포경고、중、저（100、10、1 mg/L）3충농도적유화산간예48 h후，분별검측간예전후흑소세포중흑소함량、락안산매활성적변화，채용류식법검측혼합배양세포중흑소전체적정황，이10 nmol/L웅과감위양성대조。결과3충농도유화산농도균가하조세포락안산매활성，병정농도의뢰성。제료1 mg/L적유화산대흑소세포흑소합성무명현작용，기여농도적유화산균가강저흑소함량。유화산동시구유억제흑소전체적작용。결론유화산가억제흑소합성급흑소전체，혹가작위일충피부미백제응용。
Objective To investigate the effect of ellagic acid on human epidermal melanocyte melanogenesis and melanin transfer, and the mechanism thereof. Methods The human melanocytes and and keratinocytes were co-cultured and purified. After passing the second generation, cells of 1∶10 ratio were inoculated into the small dish (3 cm × 3 cm). The changes of melanin content and tyrosinase activity in melanocytes were detected before and after intervention with ellagic acid (100, 10 and 1 mg/L) for 48 h. The melanin transfer in cultured cells was detected by flow cytometry method. The 10 nmol/L arbutin was used as the positive control. Results The tyrosinase activity was down-regulated by ellagic acid in a dose-dependent manner. The ellagic acid can reduce the melanin content except for the 1 mg/L of ellagic acid. The melanin transfer was also inhibited by ellagic acid in a dose-dependence manner. Conclusion Ellagic acid can be used for skin-whitening cosmetic and the depigmenting effect might be due to the down-regulation of melanogenesis and melanin transfer.