CAJ | 학술논문

目的：研究ALOX12基因位点rs1126667（R261Q）单核苷酸多态性（SNP）与2型糖尿病（T2DM）及糖尿病肾病（DN）的相关性。方法在北方汉族人群中采用病例-对照方法选择223例T2DM患者（其中DN 134例、T2DM无肾病89例）和120名健康体检者（正常对照组）。应用单碱基延伸反应和基质辅助激光解吸电离飞行时间质谱技术对该基因SNP进行研究。结果 ALOX12基因rs1126667位点符合Hardy-Weinberg平衡定律。正常对照组G/G、G/A、A/A 3种基因型的频率为26％、47％和27％；T2DM组的频率分别为30％、48％和22％，相对危险度（OR）分别为1、0．8、0．76；DN组的频率分别为31％、45％和24％，OR值分别为1、0．88、0．94；T2DM无肾病组的频率分别为28％、54％和18％，OR值分别为1、1．06、0．61，各组间差异均无统计学意义（P＞0．05）。正常对照组、T2DM组、DN组和T2DM无肾病组A等位基因频率分别为50％、46％、47％、45％，各组间差异无统计学意义（P＞0．05）。结论在北方汉族人群中未发现ALOX12基因多态性与T2DM、DN有关。
목적：연구ALOX12기인위점rs1126667（R261Q）단핵감산다태성（SNP）여2형당뇨병（T2DM）급당뇨병신병（DN）적상관성。방법재북방한족인군중채용병례-대조방법선택223례T2DM환자（기중DN 134례、T2DM무신병89례）화120명건강체검자（정상대조조）。응용단감기연신반응화기질보조격광해흡전리비행시간질보기술대해기인SNP진행연구。결과 ALOX12기인rs1126667위점부합Hardy-Weinberg평형정률。정상대조조G/G、G/A、A/A 3충기인형적빈솔위26％、47％화27％；T2DM조적빈솔분별위30％、48％화22％，상대위험도（OR）분별위1、0．8、0．76；DN조적빈솔분별위31％、45％화24％，OR치분별위1、0．88、0．94；T2DM무신병조적빈솔분별위28％、54％화18％，OR치분별위1、1．06、0．61，각조간차이균무통계학의의（P＞0．05）。정상대조조、T2DM조、DN조화T2DM무신병조A등위기인빈솔분별위50％、46％、47％、45％，각조간차이무통계학의의（P＞0．05）。결론재북방한족인군중미발현ALOX12기인다태성여T2DM、DN유관。
Objective To investigate the correlation of a single nucleotide polymorphism (SNP)locus rs1 126667 (R261Q)in the arachidonate 12-lipoxygenase (ALOX12)gene with type 2 diabetes mellitus(T2DM)and diabetic nephropathy(DN).Methods The SNP of ALOX12 gene was studied using a case-control method by single base extension reactions and matrix-assisted laser desorption ionization-time of flight mass spectrometry platform.A case-control study enrolled 223 T2DM patients [DN group (134 patients)and T2DM without nephropathy group (89 patients)]and 120 healthy subjects (healthy control group).Results ALOX12 gene locus rs1 126667 was consistent with Hardy-Weinberg equilibrium law,and the frequencies of ALOX12 gene locus rs1 126667 G/G,G/A and A/A genotypes in the healthy control group were 26%,47%and 27%.The frequencies in T2DMgroup were 30%,48%and the 22%,and odd ratios (OR)were 1,0.8 and 0.76.The frequencies in DN group were 31%,45% and 24%,and OR were 1,0.88 and 0.94.The frequencies in T2DMwithout nephropathy group were 28%,54%and 18%,and OR were 1,1.06 and 0.61.No statistical significance in all groups was found(P>0.05).A allele frequencies in the healthy control group,T2DMgroup,DN group and T2DMwithout nephropathy group were 50%,46%,47%and 45%without statistical significance(P>0.05).Conclusions No correlation of the ALOX12 gene polymorphism with T2DM or DM is found in the northeast Han Chinese.