CAJ | 학술논문

目的：探讨KAI1基因和基质金属蛋白酶抑制剂1（TIMP-1）、基质金属蛋白酶9（MMP-9）在非小细胞肺癌（NSCLC）中的表达，分析其与临床生物学行为的关系。方法应用免疫组化法检测73例NSCLC、51例相应的癌旁组织和13例非肿瘤性肺组织的KAI1和TIMP-1、MMP-9的表达，采用χ2检验和Pearson法分析三者与临床生物学行为的相关性。结果在73例NSCLC和64例癌旁及非肿瘤组织中，KAI1和TIMP-1的阳性率分别为39．7％、74．0％和90．6％、37．5％，两者比较差异有统计学意义（P＜0．05），而 MMP-9阳性率为79．4％和67．1％，两者比较差异无统计学意义（P＞0．05）。在NSCLC组织中，KAI1检出率在不同TNM分期、淋巴结有无转移、不同肿瘤类型中差异有统计学意义（P＜0．05），TIMP-1、MMP-9的检出率在不同TNM分期、淋巴结有无转移中差异有统计学意义（P＜0．05），三者检出率与年龄、性别无关；KAI1的表达与TIMP-1、MMP-9呈负相关（r＝-0．4637、-0．3445，P＜0．05）。结论 KAI1和TIMP-1、MMP-9均与肺癌的发展、转移有关，联合检测三者可成为评价NSCLC发生、发展的预后参考指标。
목적：탐토KAI1기인화기질금속단백매억제제1（TIMP-1）、기질금속단백매9（MMP-9）재비소세포폐암（NSCLC）중적표체，분석기여림상생물학행위적관계。방법응용면역조화법검측73례NSCLC、51례상응적암방조직화13례비종류성폐조직적KAI1화TIMP-1、MMP-9적표체，채용χ2검험화Pearson법분석삼자여림상생물학행위적상관성。결과재73례NSCLC화64례암방급비종류조직중，KAI1화TIMP-1적양성솔분별위39．7％、74．0％화90．6％、37．5％，량자비교차이유통계학의의（P＜0．05），이 MMP-9양성솔위79．4％화67．1％，량자비교차이무통계학의의（P＞0．05）。재NSCLC조직중，KAI1검출솔재불동TNM분기、림파결유무전이、불동종류류형중차이유통계학의의（P＜0．05），TIMP-1、MMP-9적검출솔재불동TNM분기、림파결유무전이중차이유통계학의의（P＜0．05），삼자검출솔여년령、성별무관；KAI1적표체여TIMP-1、MMP-9정부상관（r＝-0．4637、-0．3445，P＜0．05）。결론 KAI1화TIMP-1、MMP-9균여폐암적발전、전이유관，연합검측삼자가성위평개NSCLC발생、발전적예후삼고지표。
Objective To investigate the expressions of KAI1 gene,tissue inhibitor 1 of metalloproteinase (TIMP-1)and matrix metalloproteinase 9 (MMP-9)in non-small cell lung cancer (NSCLC)and their correlations with clinicopathologic features of NSCLC.Methods The expressions of KAI1 ,TIMP-1 and MMP-9 in 73 specimens of NSCLC tissues,51 specimens of corresponding adjacent tissues and 13 specimens of non-cancerous lung tissue were determined by immunohistochemistry.Their correlations with clinicopathologic features were analyzed by χ2 test and Pearson analysis.Results In 73 specimens of NSCLC tissues and 64 cases of corresponding adjacent and non-cancerous lung tissues,the positive rates of KAI1 and TIMP-1 were 39.7%,74.0% and 90.6%,37.5% with statistical significance (P <0.05 ),and the positive rates of MMP-9 were 79.4%,67.1% without statistical significance (P>0.05 ).The expression of KAI1 was correlated to TNM stage,lymph node metastasis,histologic subtype and differentiation with statistical significance (P<0.05),and the TIMP-1 and MMP-9 were correlated to TNM stage and lymph node metastasis (P<0.05),but they were not correlated to age and sex.The expression of KAI1 was negatively correlated with the expressions of TIMP-1 and MMP-9 (r=-0.463 7,-0.344 5,P<0.05).Conclusions The KAI1 ,TIMP-1 and MMP-9 are related to the development of lung cancer.The combination determination of them will be the important prognosis reference indicator for the occurrence and development of NSCLS.