CAJ | 학술논문

目的：利用流式细胞微球芯片捕获技术（CBA）检测慢加急性乙型肝炎肝衰竭（ACHBLF）患者辅助性T细胞1/2（Th1/Th2）分泌的细胞因子水平，探讨其在ACHBLF中变化的意义。方法收集33例ACHBLF、20例慢性乙型肝炎（CHB）患者血清及15名健康志愿者血清，CBA检测血清Th1/Th2细胞因子γ干扰素（IFN-γ）、白细胞介素2（IL-2）、白细胞介素4（IL-4）、白细胞介素6（IL-6）、白细胞介素10（IL-10）及肿瘤坏死因子α（TNF-α）水平，分析Th1/Th2细胞因子在ACHBLF患者血清中的变化。结果 ACHBLF患者外周血循环中TNF-α、IFN-γ、IL-4及IL-6的水平较CHB患者及健康对照者显著升高（P＜0．05）；ACHBLF患者血清中IL-2及IL-10与CHB组和健康对照组比较，差异无统计学意义（P＞0．05）。ACHBLF患者血清中IL-4、IL-6、TNF-α及IFN-γ水平与总胆红素（TBil）、白蛋白（Alb）和凝血酶原活动度（PTA）间无明显相关性。结论 CBA可同时检测各种细胞因子，适合临床应用；ACHBLF患者TNF-α、IFN-γ、IL-4和IL-6的变化可能参与ACHBLF的发生和发展，未发现上述细胞因子与反映肝功能指标间的相关关系。
목적：이용류식세포미구심편포획기술（CBA）검측만가급성을형간염간쇠갈（ACHBLF）환자보조성T세포1/2（Th1/Th2）분비적세포인자수평，탐토기재ACHBLF중변화적의의。방법수집33례ACHBLF、20례만성을형간염（CHB）환자혈청급15명건강지원자혈청，CBA검측혈청Th1/Th2세포인자γ간우소（IFN-γ）、백세포개소2（IL-2）、백세포개소4（IL-4）、백세포개소6（IL-6）、백세포개소10（IL-10）급종류배사인자α（TNF-α）수평，분석Th1/Th2세포인자재ACHBLF환자혈청중적변화。결과 ACHBLF환자외주혈순배중TNF-α、IFN-γ、IL-4급IL-6적수평교CHB환자급건강대조자현저승고（P＜0．05）；ACHBLF환자혈청중IL-2급IL-10여CHB조화건강대조조비교，차이무통계학의의（P＞0．05）。ACHBLF환자혈청중IL-4、IL-6、TNF-α급IFN-γ수평여총담홍소（TBil）、백단백（Alb）화응혈매원활동도（PTA）간무명현상관성。결론 CBA가동시검측각충세포인자，괄합림상응용；ACHBLF환자TNF-α、IFN-γ、IL-4화IL-6적변화가능삼여ACHBLF적발생화발전，미발현상술세포인자여반영간공능지표간적상관관계。
Objective To investigate the cytokine levels of T helper cell 1/2 (Th1/Th2)detected by cytometric bead array (CBA)in patients with acute-on-chronic hepatitis B liver failure (ACHBLF),and their change significance in ACHBLF.Methods The Th1/Th2 cytokine levels in 33 patients with ACHBLF and 20 patients with chronic hepatitis B (CHB)were detected by CBA.A total of 15 healthy subjects were enrolled as controls.The serum interferon gamma (IFN),interleukin 2 (IL-2),interleukin 4 (IL-4),interleukin 6 (IL-6),interleukin 10 (IL-10)and tumor necrosis factor alpha (TNF-α) levels were detected,and the changes of Th1/Th2 cytokine levels were analyzed. Results The serum TNF-α,IFN-γ,IL-4 and IL-6 levels of ACHBLF group were significantly higher than those of CHB and control groups (P<0.05 ).The serum IL-2 and IL-10 had no statistical significance between ACHBLF and CHB groups and between ACHBLF and control groups (P>0.05).There was no significant correlation of IL-4,IL-6,TNF-αin ACHBLF group with total bilirubin (TBil),albumin (Alb)and prothrombin activity (PTA).Conclusions CBA can determine a variety of cytokines simultaneously and is suitable for the clinical application.The changes of TNF-α, IFN-γ,IL-4 and IL-6 will involve to the pathogenesis and development of ACHBLF.There is not found the relationship between cytokines and liver function.