检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
3期
262-265
,共4页
王也飞%周怡%丁磊%夏文权%胡翊群
王也飛%週怡%丁磊%夏文權%鬍翊群
왕야비%주이%정뢰%하문권%호익군
Sysmex XN-1000全自动血液分析仪%有核红细胞%准确性
Sysmex XN-1000全自動血液分析儀%有覈紅細胞%準確性
Sysmex XN-1000전자동혈액분석의%유핵홍세포%준학성
Sysmex XN-1000 automatic hematology analyzer%Nucleated red blood cell%Accuracy
目的:探讨SYSMEX XN-1000全自动血液分析仪(简称XN-1000)计数有核红细胞(NRBC)的准确性。方法应用XN-1000对66例外周血标本进行NRBC检测,同时用流式细胞仪(FCM)和显微镜(镜检法)对同一份外周血的NRBC百分率进行计数。以镜检法为金标准,比较不同方法的计数结果。结果 XN-1000与镜检法检测NRBC有较好的相关性(r=0.927),但低于FCM与镜检法NRBC的相关性(r=0.956),XN-1000与FCM检测NRBC的相关性较高(r=0.994)。XN-1000、FCM计数结果与镜检法的结果间差异均无统计学意义(P>0.05)。XN-1000检测NRBC%较FCM法有更高的诊断特异性(95.7%)。结论 XN-1000能快速、准确、有效地自动计数外周血NRBC。
目的:探討SYSMEX XN-1000全自動血液分析儀(簡稱XN-1000)計數有覈紅細胞(NRBC)的準確性。方法應用XN-1000對66例外週血標本進行NRBC檢測,同時用流式細胞儀(FCM)和顯微鏡(鏡檢法)對同一份外週血的NRBC百分率進行計數。以鏡檢法為金標準,比較不同方法的計數結果。結果 XN-1000與鏡檢法檢測NRBC有較好的相關性(r=0.927),但低于FCM與鏡檢法NRBC的相關性(r=0.956),XN-1000與FCM檢測NRBC的相關性較高(r=0.994)。XN-1000、FCM計數結果與鏡檢法的結果間差異均無統計學意義(P>0.05)。XN-1000檢測NRBC%較FCM法有更高的診斷特異性(95.7%)。結論 XN-1000能快速、準確、有效地自動計數外週血NRBC。
목적:탐토SYSMEX XN-1000전자동혈액분석의(간칭XN-1000)계수유핵홍세포(NRBC)적준학성。방법응용XN-1000대66예외주혈표본진행NRBC검측,동시용류식세포의(FCM)화현미경(경검법)대동일빈외주혈적NRBC백분솔진행계수。이경검법위금표준,비교불동방법적계수결과。결과 XN-1000여경검법검측NRBC유교호적상관성(r=0.927),단저우FCM여경검법NRBC적상관성(r=0.956),XN-1000여FCM검측NRBC적상관성교고(r=0.994)。XN-1000、FCM계수결과여경검법적결과간차이균무통계학의의(P>0.05)。XN-1000검측NRBC%교FCM법유경고적진단특이성(95.7%)。결론 XN-1000능쾌속、준학、유효지자동계수외주혈NRBC。
Objective To investigate the accuracy of nucleated red blood cell (NRBC)count determined by SYSMEX XN-1000 automatic hematology analyzer(XN-1000).Methods A total of 66 peripheral blood samples were collected and determined for NRBC by XN-1000,and the results of NRBC%were compared with those by flow cytometry (FCM)and manual microscopy for the same sample.The manual microscopy was as golden standard.Results There was a good correlation for NRBC between XN-1000 and microscopy (r =0.927 ),but the correlation was lower than that between FCMand manual microscopy (r=0.956).The correlation between XN-1000 and FCMwas the best (r=0.994). The results of XN-1000 and FCMwith manual microscopy had no statistical significance(P>0.05).XN-1000 for NRBC%had a better specificity (95.7%) than FCM.Conclusions XN-1000 automatic hematology analyzer has good performance for detecting NRBC in peripheral blood rapidly,accurately and effectively.