检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2014年
3期
258-261
,共4页
马晓萍%高晓勤%杨燕平%杨喆
馬曉萍%高曉勤%楊燕平%楊喆
마효평%고효근%양연평%양철
顶体蛋白酶%透明质酸酶%生育力%男性
頂體蛋白酶%透明質痠酶%生育力%男性
정체단백매%투명질산매%생육력%남성
Acrosome enzyme%Hyaluronidase%Fertility%Male
目的：探讨人精子顶体内酶对男性生育力的影响。方法参照世界卫生组织标准方法对52例不育症患者、12名正常生育者[正常生育组，精子密度为（50～100）×109/L、精子活动率为60％～90％]进行精液常规分析。不育组按精子密度不同分为B1（＜20×109/L）、C1（20×109～40×109/L）及D1（＞40×109/L）3组；按精子活动率不同分为B2（＜30％）、C2（30％～50％）及D2（＞50％）3组]。采用改良明胶底物膜法和改良透明质酸钠-明胶底物膜法分别检测精子顶体内顶体蛋白酶（ACE）活性（ACE阳性反应率、ACE活性强度）和透明质酸酶（HYD）活性（HYD阳性反应率、HYD活性强度）。结果精子ACE活性（ACE阳性反应率、ACE活性强度）与精子密度、活动率、形态畸形率明显相关[相关系数（r）分别为0．797、0．867，0．831、0．860和-0．625、-0．546， P均＜0．01]；精子HYD活性（HYD阳性反应率、HYD活性强度）与精子密度、活动率、形态畸形率明显相关[r值分别为0．832、0．855，0．842、0．830和-0．625、-0．554，P均＜0．01]。不育各组精子形态畸形率明显高于正常生育组（P＜0．01）。C1组、D1组和 C2组、D2组 ACE 活性（ACE 阳性反应率、ACE 活性强度）、HYD 活性（HYD阳性反应率、HYD活性强度）明显低于正常生育组（P＜0．01）。各组酶活性随精子密度及活动率增加而上升。结论精子ACE、HYD活性的检测可作为一种有效的判断生育力的检测指标。
目的：探討人精子頂體內酶對男性生育力的影響。方法參照世界衛生組織標準方法對52例不育癥患者、12名正常生育者[正常生育組，精子密度為（50～100）×109/L、精子活動率為60％～90％]進行精液常規分析。不育組按精子密度不同分為B1（＜20×109/L）、C1（20×109～40×109/L）及D1（＞40×109/L）3組；按精子活動率不同分為B2（＜30％）、C2（30％～50％）及D2（＞50％）3組]。採用改良明膠底物膜法和改良透明質痠鈉-明膠底物膜法分彆檢測精子頂體內頂體蛋白酶（ACE）活性（ACE暘性反應率、ACE活性彊度）和透明質痠酶（HYD）活性（HYD暘性反應率、HYD活性彊度）。結果精子ACE活性（ACE暘性反應率、ACE活性彊度）與精子密度、活動率、形態畸形率明顯相關[相關繫數（r）分彆為0．797、0．867，0．831、0．860和-0．625、-0．546， P均＜0．01]；精子HYD活性（HYD暘性反應率、HYD活性彊度）與精子密度、活動率、形態畸形率明顯相關[r值分彆為0．832、0．855，0．842、0．830和-0．625、-0．554，P均＜0．01]。不育各組精子形態畸形率明顯高于正常生育組（P＜0．01）。C1組、D1組和 C2組、D2組 ACE 活性（ACE 暘性反應率、ACE 活性彊度）、HYD 活性（HYD暘性反應率、HYD活性彊度）明顯低于正常生育組（P＜0．01）。各組酶活性隨精子密度及活動率增加而上升。結論精子ACE、HYD活性的檢測可作為一種有效的判斷生育力的檢測指標。
목적：탐토인정자정체내매대남성생육력적영향。방법삼조세계위생조직표준방법대52례불육증환자、12명정상생육자[정상생육조，정자밀도위（50～100）×109/L、정자활동솔위60％～90％]진행정액상규분석。불육조안정자밀도불동분위B1（＜20×109/L）、C1（20×109～40×109/L）급D1（＞40×109/L）3조；안정자활동솔불동분위B2（＜30％）、C2（30％～50％）급D2（＞50％）3조]。채용개량명효저물막법화개량투명질산납-명효저물막법분별검측정자정체내정체단백매（ACE）활성（ACE양성반응솔、ACE활성강도）화투명질산매（HYD）활성（HYD양성반응솔、HYD활성강도）。결과정자ACE활성（ACE양성반응솔、ACE활성강도）여정자밀도、활동솔、형태기형솔명현상관[상관계수（r）분별위0．797、0．867，0．831、0．860화-0．625、-0．546， P균＜0．01]；정자HYD활성（HYD양성반응솔、HYD활성강도）여정자밀도、활동솔、형태기형솔명현상관[r치분별위0．832、0．855，0．842、0．830화-0．625、-0．554，P균＜0．01]。불육각조정자형태기형솔명현고우정상생육조（P＜0．01）。C1조、D1조화 C2조、D2조 ACE 활성（ACE 양성반응솔、ACE 활성강도）、HYD 활성（HYD양성반응솔、HYD활성강도）명현저우정상생육조（P＜0．01）。각조매활성수정자밀도급활동솔증가이상승。결론정자ACE、HYD활성적검측가작위일충유효적판단생육력적검측지표。
Objective To investigate the influence of enzymes in human sperm acrosome on male fertility. Methods According to the World Health Organization standard method,52 cases were enrolled as infertile group,and 12 cases were enrolled as normal control group[sperm density (50-100)×109/L and sperm motility 60%-90%],and semen routine analysis was performed.According to the density of sperm,the infertile group was classified into infertile group B1(<20 ×109/L),C1(20 ×109-40 ×109/L)and D1(>40 ×109/L),and according to the motility of sperm, the infertile group was classified into infertile group B2 (<30%),C2 (30%-50%)and D2 (>50%).The positive reaction rate and activity intensity of acrosome enzyme (ACE)were detected by fixed gelatin substrate film technique . The positive reaction rate and activity intensity of hyaluronidase (HYD)were detected by modified sodium hyaluronate-gelatin membrane.Results There was a significant correlation between the positive reaction rate and activity intensity of ACE with sperm density,sperm motility and sperm deformity rate [correlation coefficients (r)were 0.797,0.867;0.831,0.860 and -0.625,-0.546,P<0.01].There was a significant correlation between the positive reaction rate and activity intensity of HYD with sperm density,sperm motility and sperm deformity rate (r were 0.832,0.855;0.842,0.830 and -0.625,-0.554,P<0.01).The sperm deformity rate in infertile group was higher than that in normal control group (P<0.01).The positive reaction rates and activity intensities of ACE and HYD in infertile group C1,D1 and C2,D2 were significantly lower than those in normal control group (P<0.01).The activity of enzymes increased with the sperm density and motility.Conclusions The detections of sperm ACE and HYD are effective parameters for the analysis of male fertility.
