临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
3期
258-260
,共3页
马荣伟%姚建宏%冀湧%王莉
馬榮偉%姚建宏%冀湧%王莉
마영위%요건굉%기용%왕리
硫酸特布他林%肺液转运%Na+,K+-ATP酶%早产大鼠
硫痠特佈他林%肺液轉運%Na+,K+-ATP酶%早產大鼠
류산특포타림%폐액전운%Na+,K+-ATP매%조산대서
terbutaline sulfate%lung fluid transport%Na+,K+-ATP enzyme%premature rat
目的：观察β2受体激动剂硫酸特布他林对早产新生大鼠肺液转运的影响及机制。方法随机将孕鼠分为5组，对照组，早产组，特布他林低、高剂量组及地塞米松组，于受孕后第16天起以灌胃方法连续3 d分别给予不同药物。对照组取足月分娩新生鼠，其余各组大鼠于孕第19天行剖宫产，取早产鼠；新生鼠处死后取肺组织，测定肺组织湿重/干重之比、Na+，K+-ATP酶活性和环磷酸腺苷（cAMP）水平。结果五组新生鼠肺组织湿重/干重、Na+，K+-ATP酶活性以及cAMP水平的差异均有统计学意义（P均<0.01）。肺组织湿重/干重，以早产组最高，对照组最低；高剂量组低于低剂量组和地塞米松组，差异有统计学意义（P均<0.05）。Na+，K+-ATP酶活性，以早产组最低，高剂量组最高；地塞米松组、低剂量组和高剂量组均较早产组升高；高剂量组高于低剂量组和地塞米松组，差异均有统计学意义（P均<0.05）。cAMP水平，以早产组最低，高剂量组最高；地塞米松组、低剂量组和高剂量组均较早产组升高；高剂量组高于低剂量组和地塞米松组，差异均有统计学意义（P均<0.05）。结论产前使用β2受体激动剂硫酸特布他林，可降低早产新生大鼠肺组织湿重/干重之比，提高肺组织cAMP水平和增加Na+，K+-ATP酶活性。
目的：觀察β2受體激動劑硫痠特佈他林對早產新生大鼠肺液轉運的影響及機製。方法隨機將孕鼠分為5組，對照組，早產組，特佈他林低、高劑量組及地塞米鬆組，于受孕後第16天起以灌胃方法連續3 d分彆給予不同藥物。對照組取足月分娩新生鼠，其餘各組大鼠于孕第19天行剖宮產，取早產鼠；新生鼠處死後取肺組織，測定肺組織濕重/榦重之比、Na+，K+-ATP酶活性和環燐痠腺苷（cAMP）水平。結果五組新生鼠肺組織濕重/榦重、Na+，K+-ATP酶活性以及cAMP水平的差異均有統計學意義（P均<0.01）。肺組織濕重/榦重，以早產組最高，對照組最低；高劑量組低于低劑量組和地塞米鬆組，差異有統計學意義（P均<0.05）。Na+，K+-ATP酶活性，以早產組最低，高劑量組最高；地塞米鬆組、低劑量組和高劑量組均較早產組升高；高劑量組高于低劑量組和地塞米鬆組，差異均有統計學意義（P均<0.05）。cAMP水平，以早產組最低，高劑量組最高；地塞米鬆組、低劑量組和高劑量組均較早產組升高；高劑量組高于低劑量組和地塞米鬆組，差異均有統計學意義（P均<0.05）。結論產前使用β2受體激動劑硫痠特佈他林，可降低早產新生大鼠肺組織濕重/榦重之比，提高肺組織cAMP水平和增加Na+，K+-ATP酶活性。
목적：관찰β2수체격동제류산특포타림대조산신생대서폐액전운적영향급궤제。방법수궤장잉서분위5조，대조조，조산조，특포타림저、고제량조급지새미송조，우수잉후제16천기이관위방법련속3 d분별급여불동약물。대조조취족월분면신생서，기여각조대서우잉제19천행부궁산，취조산서；신생서처사후취폐조직，측정폐조직습중/간중지비、Na+，K+-ATP매활성화배린산선감（cAMP）수평。결과오조신생서폐조직습중/간중、Na+，K+-ATP매활성이급cAMP수평적차이균유통계학의의（P균<0.01）。폐조직습중/간중，이조산조최고，대조조최저；고제량조저우저제량조화지새미송조，차이유통계학의의（P균<0.05）。Na+，K+-ATP매활성，이조산조최저，고제량조최고；지새미송조、저제량조화고제량조균교조산조승고；고제량조고우저제량조화지새미송조，차이균유통계학의의（P균<0.05）。cAMP수평，이조산조최저，고제량조최고；지새미송조、저제량조화고제량조균교조산조승고；고제량조고우저제량조화지새미송조，차이균유통계학의의（P균<0.05）。결론산전사용β2수체격동제류산특포타림，가강저조산신생대서폐조직습중/간중지비，제고폐조직cAMP수평화증가Na+，K+-ATP매활성。
Objectives To investigate the effect and mechanism of terbutaline sulfate on pulmonary fluid transport in pre-mature rats. Methods Pregnant rats were randomly divided into 5 groups (control group, premature group, low-dose terbutaline group, high-dose terbutaline group and dexamethasone group). Drugs were administered by gavage after rats were fertilized for 16 days and continued for 3 days. Premature rats were taken out from the 19 days pregnant rats, and mature rats were delivered on the due day. Lungs were collected, and the ratio of pulmonary wet weight to dry weight (W/D), Na+, K+-ATP ase activity and concentration of cyclic adenosine monophosphate (cAMP) were measured in lungs. Results The W/D rate, Na+,K+-ATPase acti-vity and cAMP concentration in lungs had significant difference among different groups (P<0.01). The W/D rate was highest in premature group and lowest in the control group. It was lower in the high-dose terbutaline group than in the low-dose terbutaline group and the dexamethasone group (P<0.05). The Na+,K+-ATPase activity was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, and high-dose terbutaline group than in premature group, and it was higher in high-dsoe terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). The cAMP levle was lowest in premature group and highest in high-dose terbutaline group. It was higher in dexamethasone group, low-dose terbutaline group, high-dose terbutaline group than in premature group, and it was higher in high-dose terbutaline group than in low-dose terbutaline group and dexamethasone group (P<0.05). Conclusions Terbutaline sulfate facilitates lung fluid transport in premature rats, leading to reduce the W/D rate in terbutaline-treated group. We speculate that this effect is related to the increased cAMP level and Na+, K+-ATPase activity in lung tissue.
