CAJ | 학술논문

目的：比较3种脂肪乳剂对脂多糖（LPS）诱导的急性肺损伤（ALI）大鼠IL-1β和IL-6分泌的影响。方法100只SD幼年大鼠分为对照组、LPS组、ω-9组、ω-6组和ω-3组。日龄28 d时连续7 d，对照组大鼠经尾静脉注射生理盐水；ω-6组注射脂肪乳(C14～24)、ω-9组注射长链脂肪乳、ω-3组注射ω-3鱼油脂肪乳注射液；随后对照组气管内滴入生理盐水，另4组大鼠均喷洒LPS溶液。8 h后处死大鼠观察各组肺组织的病理改变，并测定IL-1β和IL-6 mRNA及蛋白等水平。结果各组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血；4组ALI大鼠的肺系数、肺损伤评分均高于对照组，差异均有统计学意义（P均<0.005）；ω-9组和ω-3组两组肺组织中IL-1β和IL-6 mRNA表达均较LPS组和ω-6组降低，差异均有统计学意义（P均<0.005）；支气管肺泡灌洗液中ω-9组和ω-3组IL-1β和IL-6蛋白水平均低于其余组，差异均有统计学意义（P均<0.005）。ω-9组和ω-3组IL-1β和IL-6 mRNA表达及其相应的蛋白水平差异均无统计学意义（P均>0.005）。结论ω-6 PUFAs可增加IL-1β和IL-6的产生加重ALI大鼠炎症反应；而ω-9 MUFAs和ω-3 PUFAs可降低IL-1β和IL-6减轻ALI大鼠炎症反应，具有一定的抗炎作用。
목적：비교3충지방유제대지다당（LPS）유도적급성폐손상（ALI）대서IL-1β화IL-6분비적영향。방법100지SD유년대서분위대조조、LPS조、ω-9조、ω-6조화ω-3조。일령28 d시련속7 d，대조조대서경미정맥주사생리염수；ω-6조주사지방유(C14～24)、ω-9조주사장련지방유、ω-3조주사ω-3어유지방유주사액；수후대조조기관내적입생리염수，령4조대서균분쇄LPS용액。8 h후처사대서관찰각조폐조직적병리개변，병측정IL-1β화IL-6 mRNA급단백등수평。결과각조대서폐조직병리절편균가견명현염증세포침윤화출혈；4조ALI대서적폐계수、폐손상평분균고우대조조，차이균유통계학의의（P균<0.005）；ω-9조화ω-3조량조폐조직중IL-1β화IL-6 mRNA표체균교LPS조화ω-6조강저，차이균유통계학의의（P균<0.005）；지기관폐포관세액중ω-9조화ω-3조IL-1β화IL-6단백수평균저우기여조，차이균유통계학의의（P균<0.005）。ω-9조화ω-3조IL-1β화IL-6 mRNA표체급기상응적단백수평차이균무통계학의의（P균>0.005）。결론ω-6 PUFAs가증가IL-1β화IL-6적산생가중ALI대서염증반응；이ω-9 MUFAs화ω-3 PUFAs가강저IL-1β화IL-6감경ALI대서염증반응，구유일정적항염작용。
Objective To compare the effect of three types of lipid emulsions on the IL-1βand IL-6 expressions in acute lung injury induced by lipopolysaccharide (LPS) in rats. Methods One hundred young SD rats were divided into control,LPS,ω-6,ω-9 andω-3 group. Age 29 days, the control group were intravenously injected with saline,ω-6 group injected lipid emu-sion (C14-24),ω-9 group injected long chain fat emusion,ω-3 group injected fish oil fat emusion. Then the control group fol-lowed by intratracheal instillation of saline, and the reamining four groups were sprayed with LPS. Pathologic changes in lung tissue section were observed. The expression levels of IL-1βmRNA and IL-6 mRNA were determined by RT-PCR,and the con-centrations of IL-1βand IL-6 in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immune-specific assay. Results Infiltration and bleeding were observed in lung tissue under light microscopy in ALI rats. The lung indexes and patho-logical scores of ALI model groups were significantly higher than those of control group (P<0.005). The expressions of IL-1βand IL-6 mRNA in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). The levels of IL-1βand IL-6 in BALF in groupsω-9 andω-3 were significantly higher than those in groups LPS andω-6 (P<0.005). There was no significant difference (P>0.005) of the expressions of IL-1βand IL-6 mRNA betweenω-9 andω-3 group. Conclusions ω-6 PUFAs can accentuate inflammation by up-regulating the levels of IL-1βand IL-6 whileω-9 PUFAs andω-3 PUFAs can relieve inflammation by down-regulating the levels of IL-1βand IL-6.