CAJ | 학술논문

本研究检测了无患子皂苷对6种典型细菌和6种典型真菌的抗菌活性。提取无患子假种皮中总皂苷R1和R2，分离得到皂苷混合物样品R1-3、R1-4、R1-5、R1-6、R1-7、R2-5、R2-6、R2-7、R2-8、R2-9和R2-10；采用滤纸片琼脂扩散法对其抑菌活性进行检测，得出抑菌圈直径、MIC值和EC50值。抑菌活性物质主要集中在样品R1-5、R1-6、R2-7、R2-8、R2-9和R2-10中，这些样品对金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌、大肠杆菌、巨大芽孢杆菌、白色念珠菌、黑曲霉和绿色木霉具有很强的抑菌活性。无患子总皂苷各组分中含有能够显著抑制供试细菌和真菌的活性物质，这为进一步开发利用无患子的药用价值提供了科学依据。
본연구검측료무환자조감대6충전형세균화6충전형진균적항균활성。제취무환자가충피중총조감R1화R2，분리득도조감혼합물양품R1-3、R1-4、R1-5、R1-6、R1-7、R2-5、R2-6、R2-7、R2-8、R2-9화R2-10；채용려지편경지확산법대기억균활성진행검측，득출억균권직경、MIC치화EC50치。억균활성물질주요집중재양품R1-5、R1-6、R2-7、R2-8、R2-9화R2-10중，저사양품대금황색포도구균、고초아포간균、사상아포간균、대장간균、거대아포간균、백색념주균、흑곡매화록색목매구유흔강적억균활성。무환자총조감각조분중함유능구현저억제공시세균화진균적활성물질，저위진일보개발이용무환자적약용개치제공료과학의거。
This research aimed to test the antimicrobial activities of saponins from Sapindus mukorossi Gaertn on 6 strains of representative bacterium and 6 strains of representative fungus. Total saponins (R1 and R2) were extracted from the arillus of S. mukorossi Gaertn by systemic solvents. Different polarity saponins R1-3, R1-4, R1-5, R1-6, R1-7, R2-5, R2-6, R2-7, R2-8, R2-9 and R2-10 were isolated by D-101 with different concentrations of ethyl alcohol. These samples were tested against 6 strains of bacterium and 6 strains of fungus using filter paper agar diffusion method. The diameter of antimicrobial rings, values of MIC and values of EC50 were obtained. The results showed that R1-5, R1-6, R2-7, R2-8, R2-9 and R2-10 had remarkable inhibitory activity against almost all the tested bacteria and fungi. These samples had intensively inhibitory activity on Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Bacillus megathrium, Candida albicans, Asperhillus niger and Trichoderma lignorum. Different samples of total saponins from S. mukorossi Gaertn had significantly inhibitory activity against the tested bacteria and fungi, these results supported the applications of using S. mukorossi Gaertn in medical fields.