江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
3期
16-17,21
,共3页
宁玉草莓%组织培养%快繁技术
寧玉草莓%組織培養%快繁技術
저옥초매%조직배양%쾌번기술
“Ningyu” strawberry%Tissue culture%Rapid propagation
以宁玉草莓匍匐茎为外植体,进行消毒灭菌、茎尖分化、继代增殖和生根培养基筛选研究,结果表明:最佳消毒方法为75%乙醇40 s+0.1%升汞6~8 min;草莓茎尖分化培养基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;继代增殖培养基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,其增殖系数为9;生根培养基:1/2MS+NAA 0.05 mg/L,生根率可达96.6%。
以寧玉草莓匍匐莖為外植體,進行消毒滅菌、莖尖分化、繼代增殖和生根培養基篩選研究,結果錶明:最佳消毒方法為75%乙醇40 s+0.1%升汞6~8 min;草莓莖尖分化培養基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;繼代增殖培養基:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,其增殖繫數為9;生根培養基:1/2MS+NAA 0.05 mg/L,生根率可達96.6%。
이저옥초매포복경위외식체,진행소독멸균、경첨분화、계대증식화생근배양기사선연구,결과표명:최가소독방법위75%을순40 s+0.1%승홍6~8 min;초매경첨분화배양기:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;계대증식배양기:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,기증식계수위9;생근배양기:1/2MS+NAA 0.05 mg/L,생근솔가체96.6%。
The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS +0.5 mg/L 6-BA+0.1 mg/L NAA;the best medium for subcultural propagation was MS +0.5 mg/L 6-BA+0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS+0.05 mg/L NAA, and the rooting rate reached 96.6%.