CAJ | 학술논문

为了研究农杆菌和质粒DNA两种子房注射法对普通棉花品种的转化效率，以8891棉花为材料，分别在三亚和常德两地进行了转化试验。结果表明：与质粒DNA注射相比，三亚地区农杆菌注射的转化效率高1.25个百分点；但农杆菌子房注射的落铃率高，获得的种子相对较少；而在不同的地点试验，常德地区的落铃率显著高于三亚地区；而质粒DNA直接注射的成铃率更高，虽然转化率相对较低，但也能获得有效的转化植株。
위료연구농간균화질립DNA량충자방주사법대보통면화품충적전화효솔，이8891면화위재료，분별재삼아화상덕량지진행료전화시험。결과표명：여질립DNA주사상비，삼아지구농간균주사적전화효솔고1.25개백분점；단농간균자방주사적락령솔고，획득적충자상대교소；이재불동적지점시험，상덕지구적락령솔현저고우삼아지구；이질립DNA직접주사적성령솔경고，수연전화솔상대교저，단야능획득유효적전화식주。
In order to study the transformation efficiency of two ovary injection methods (with Agrobacterium tumefaciens or with plasmid DNA) to normal cotton variety 8891, the transformation experiments were conducted in Sanya and Changde, respectively. The results showed that the ovary injection with Agrobacterium tumefaciens in Sanya increased the transformation efficiency by 1.25 percentage points, but it had relatively higher boll shedding rate which causing relatively less seeds, moreover, the boll shedding rate in Changde was significantly higher than that in Sanya; ovary injection with plasmid DNA had relatively higher boll setting rate, and it can obtain the effective transformation plant although the transformation efficiency was relatively lower.