CAJ | 학술논문

目的：检测淋巴瘤组织中4-1BBL的表达水平，并分析其临床意义。方法对80例淋巴瘤组织芯片，采用免疫组织化学的方法，采用不同抗体量化测定其CD20、4-1BBL N端和4-1BBL C端表达情况；并且应用Image-Pro Plus软件计算各病例的IOD值。结果霍奇金病（ HD）患者中CD20抗原表达水平显著高于非霍奇金淋巴瘤（ NHL）患者；N端和C端4-1BBL表达水平并无显著相关性，不同患者4-1BBL转变为s4-1BBL的比率不同；通过测定IOD值发现大多数s4-1BBL是通过MMP直接切割导致的；少部分s4-1BBL是直接表达出来转运到细胞外的。结论不同淋巴瘤患者4-1 BBL的表达水平不同、S4-1 BBL的产生机制也不同，针对不同患者应提供不同的免疫治疗策略。
목적：검측림파류조직중4-1BBL적표체수평，병분석기림상의의。방법대80례림파류조직심편，채용면역조직화학적방법，채용불동항체양화측정기CD20、4-1BBL N단화4-1BBL C단표체정황；병차응용Image-Pro Plus연건계산각병례적IOD치。결과곽기금병（ HD）환자중CD20항원표체수평현저고우비곽기금림파류（ NHL）환자；N단화C단4-1BBL표체수평병무현저상관성，불동환자4-1BBL전변위s4-1BBL적비솔불동；통과측정IOD치발현대다수s4-1BBL시통과MMP직접절할도치적；소부분s4-1BBL시직접표체출래전운도세포외적。결론불동림파류환자4-1 BBL적표체수평불동、S4-1 BBL적산생궤제야불동，침대불동환자응제공불동적면역치료책략。
Objective To detect expression of 4-1BBL in lymphoma and its clinical significance .Methods Immunohis-tochemistry was performed in 80 cases of lymphoma tissue microarrays ,and expression of CD20,C-terminus and N-terminus of 4-1BBL were detected by antibody quantification .Integrated optical density ( IOD) was calculated using Image-Pro plus software. Results Expression of CD20 in Hodgkin’s disease was higher than that of Non-Hodgkin’s lymphoma.Expression of 4-1BBL be-tween C-terminus and N-terminus had no significant correlations .The transformation ratio of s4-1BBL into 4-1BBL was different in different patients.IOD showed that a large proportion of s4-1BBL was derived from matrix metalloproteinase mechanism ,and an-other small proportion of s4-1BBL was directly expressed and transported extracellular .Conclusion The expression of 4-1BBL and generation of s4-1BBL in different patients are different .Different immunotherapy strategies should be provided to different patients.