CAJ | 학술논문

目的：建立6-氨基喹啉基-N-羟基丁二酰亚胺基-氨基甲酸酯（AQC）柱前衍生-超高效液相色谱法测定人体血清中17种游离氨基酸。方法血清样品经乙腈沉淀蛋白后，在硼酸盐缓冲液中，游离氨基酸与A QC反应生成结构稳定的有紫外吸收的衍生物，用反相C18柱分离，紫外检测器检测，外标法定量分析。结果17种游离氨基酸的批内精密度范围为2．81％～7．35％，批间精密度范围为7．44％～15．40％，在2．5～250μmol/L范围线性良好，线性相关系数为0．9960～1．0000，相对标准偏差小于3％。结论该方法测定血清中17种游离氨基酸，10 min能够完全分离，结果准确、稳定、高效。
목적：건립6-안기규람기-N-간기정이선아알기-안기갑산지（AQC）주전연생-초고효액상색보법측정인체혈청중17충유리안기산。방법혈청양품경을정침정단백후，재붕산염완충액중，유리안기산여A QC반응생성결구은정적유자외흡수적연생물，용반상C18주분리，자외검측기검측，외표법정량분석。결과17충유리안기산적비내정밀도범위위2．81％～7．35％，비간정밀도범위위7．44％～15．40％，재2．5～250μmol/L범위선성량호，선성상관계수위0．9960～1．0000，상대표준편차소우3％。결론해방법측정혈청중17충유리안기산，10 min능구완전분리，결과준학、은정、고효。
Objective To establish a method for the determination of 17 kinds of free amino acid in serum by the ultra perform-ance liquid chromatography(UPLC) with AQC pre-column derivatization .Methods After proteins precipitation in serum sample by acetonitrile ,free amino acids with aminoquinoline-N-butyl hydroxy diimide and carbamate(AQC) were reacted to generate the struc-tural stable derivative products with ultraviolet absorption .These products were separated by reversed-phase C18 column ,detected with UV detector and quantitated by the external standard .Results The within-run precision of 17 kinds of free amino acid was 2 . 81% -7 .35% and the between-run precision was 7 .44% -15 .40% .The linear relationship was good in the range of 2 .5-250μmol/L ,the linear correlation coefficient was 0 .9960 -1 .0000 ,RSD<3% .Conclusion Using this method for determining 17 kinds of amino acid in serum ,the complete separation is obtained within 10 min ,the results are accurate ,stable and highly efficient .