CAJ | 학술논문

目的：了解药物维生素C（VitC）、氯化钾（KCl）及止血敏对盐水介质、微柱凝胶配血法的影响，并与凝聚胺配血法对比。方法分别在含与不含有特异性抗体两种条件下，加入KCI注射液、酚磺乙胺（止血敏）注射液、VitC注射液、生理盐水，与相应红细胞进行免疫学反应。观察3种药物对不同介质配血方法的影响。结果低浓度的VitC、KCl不影响凝聚胺介质配血，止血敏令试验产生假凝集；高浓度的3种药物可导致试验失败。盐水介质法检测敏感性略低，VitC、KCl对试验的影响不明显，而KCl组随着抗体被稀释，凝集强度减弱甚至于消失。结论3种药物对微柱凝胶配血法均有影响，尤其受KCl影响较大。
목적：료해약물유생소C（VitC）、록화갑（KCl）급지혈민대염수개질、미주응효배혈법적영향，병여응취알배혈법대비。방법분별재함여불함유특이성항체량충조건하，가입KCI주사액、분광을알（지혈민）주사액、VitC주사액、생리염수，여상응홍세포진행면역학반응。관찰3충약물대불동개질배혈방법적영향。결과저농도적VitC、KCl불영향응취알개질배혈，지혈민령시험산생가응집；고농도적3충약물가도치시험실패。염수개질법검측민감성략저，VitC、KCl대시험적영향불명현，이KCl조수착항체피희석，응집강도감약심지우소실。결론3충약물대미주응효배혈법균유영향，우기수KCl영향교대。
Objective To comprehend the influences of vitamin C ,KCL and dicynone on blood cross matching of saline and mi-crocolumn gel and to compare them with polybrene crossing matching .Methods Under the conditions with and without the specific antibody ,KCI injection ,dicynone injection ,vitamin C injection and normal saline were added for conducting the immunological reac-tion with corresponding RBC .The influences of 3 kinds of drug on different medium cross matching methods of different mediums . Results The low concentration of vitamin C and KCL does not affect polybrene cross matching ,dicynone makes the experiment to generate pseudoagglutination ;the high concentration of these 3 kinds of drug can cause the test to fail .The sensitivity of the saline medium method is slightly lower ,the influence of vitamin C and KCL on the test is inapparent ,while in the KCL method ,with the dilution of antibody ,the agglutination intensity is weakened and even disappeared .Conclusion 3 kinds of drug all have influences on the mierocolumn gel cross matching method ,especially KCL .