CAJ | 학술논문

目的：通过定量检测肝细胞癌、慢性肝炎肝硬化患者及健康者血清中高尔基体蛋白73（GP73）和甲胎蛋白（AFP）水平，对比分析GP73与AFP的检出率，探讨GP73用于诊断肝细胞癌的临床意义。方法收集健康者、慢性肝炎肝硬化及肝细胞癌患者血清样本，分别采用酶联免疫吸附测定法和化学发光法检测GP73和 AFP。结果在肝细胞癌患者血清中，GP73检出率为75．61％，AFP检出率为40．24％，GP73明显高于AFP ，差异有统计学意义（P＜0．01）；慢性肝炎肝硬化患者GP73检出率为12．58％，AFP检出率为41．67％，GP73检出率明显低于 AFP ，差异有统计学意义（P＜0．01）；健康者GP73检出率为1．67％， AFP检出率为6．67％，两者比较差异无统计学意义（P＞0．05）。在健康者和慢性肝炎肝硬化患者中GP73特异度为93．42％， AFP特异度为77．78％；ROC曲线分析显示GP73和AFP的曲线下面积分别为0．9341和0．8066，GP73与AFP两者联合检测对肝细胞癌的检出率为87．80％。结论 G P73在健康者中检出率和水平极低，慢性肝炎肝硬化患者为12．58％，肝细胞癌患者高达75．61％。ROC曲线分析与检出率基本一致。GP73在肝细胞癌患者中检出率高，特异度强，是早期诊断肝细胞癌的首选标志物。联合GP73和AFP检测可以提高对肝细胞癌的早期诊断率。
목적：통과정량검측간세포암、만성간염간경화환자급건강자혈청중고이기체단백73（GP73）화갑태단백（AFP）수평，대비분석GP73여AFP적검출솔，탐토GP73용우진단간세포암적림상의의。방법수집건강자、만성간염간경화급간세포암환자혈청양본，분별채용매련면역흡부측정법화화학발광법검측GP73화 AFP。결과재간세포암환자혈청중，GP73검출솔위75．61％，AFP검출솔위40．24％，GP73명현고우AFP ，차이유통계학의의（P＜0．01）；만성간염간경화환자GP73검출솔위12．58％，AFP검출솔위41．67％，GP73검출솔명현저우 AFP ，차이유통계학의의（P＜0．01）；건강자GP73검출솔위1．67％， AFP검출솔위6．67％，량자비교차이무통계학의의（P＞0．05）。재건강자화만성간염간경화환자중GP73특이도위93．42％， AFP특이도위77．78％；ROC곡선분석현시GP73화AFP적곡선하면적분별위0．9341화0．8066，GP73여AFP량자연합검측대간세포암적검출솔위87．80％。결론 G P73재건강자중검출솔화수평겁저，만성간염간경화환자위12．58％，간세포암환자고체75．61％。ROC곡선분석여검출솔기본일치。GP73재간세포암환자중검출솔고，특이도강，시조기진단간세포암적수선표지물。연합GP73화AFP검측가이제고대간세포암적조기진단솔。
Objective To quantitatively detect serum Golgi protein 73 (GP73) and alpha-fetoprotein (AFP) in the patients with hepatocellular carcinoma (HCC) ,chronic hepatitis liver cirrhosis and normal healthy individuals ,to compare the detection rate of GP73 and AFP and to investigate the clinical significance of GP73 for diagnosing HCC .Methods The serum samples in normal healthy individuals and the patients with chronic hepatitis liver cirrhosis and HCC were collected and detected GP 73 and AFP by the enzyme-linked immunosorbent assay (ELISA) and the chemiluminescence method .Results The detection rates of GP73 and AFP in HCC were 75 .61% and 40 .24% respectively ,GP73 was significantly higher than AFP (P<0 .01);In chronic hepatitis liver cirrhosis ,the detection rates of GP73 and AFP were 12 .58% and 41 .67% respectively ,the detection rate of GP73 was significantly lower than that of AFP (P<0 .01);In normal healthy individuals ,the detection rates of GP73 and AFP were 1 .67% and 6 .67% re-spectively ,the difference between them had no statistical significance (P>0 .05) .The specificity of GP73 and AFP in the normal healthy individuals and the patients with chronic hepatitis kive cirrhosis were 93 .42% and 77 .78% respectively .Areas under the ROC curves of GP73 and AFP were 0 .934 1 and 0 .806 6 respectively .The HCC detection rate of the combined detection of GP73 and AFPT was 87 .80% .Conclusion The GP73 detection rate and content are extremely low in the normal healthy individuals ,the GP73 detection rate is 12 .58% in chronic hepatitis liver cirrhosis and up to 75 .6% in HCC .The ROC curve analysis and the detec-tion rate are basically consistent .GP73 has the high detection rate and strong specificity in the patients with HCC ,which is the pre-ferred marker for early diagnosis of HCC .The joint detection of GP73 and AFP can improve the early diagnosis rate of HCC .