CAJ | 학술논문

目的：比较荧光定量聚合酶链反应法（简称荧光定量法）与基因芯片分型法（简称基因芯片法）在检测人乳头瘤病毒（H PV ）中的敏感度。方法选取2010年2月至2013年2月来该院妇科门诊就诊的患者305例，收集其宫颈脱落细胞，分别用荧光定量法和基因芯片法检测 H PV ，对结果不一致的病例采用测序法进行确证。305例病例均进行液基细胞学检测。结果荧光定量法检测 HPV敏感度为33．8％，基因芯片法检测HPV敏感度为37．6％，两种方法具有较高的一致性，HPV感染率随宫颈脱落细胞异型性增加而增加。结论荧光定量法和基因芯片法检测 H PV具有较高的一致性，其中基因芯片法敏感度较高。宫颈细胞学改变的严重程度与 HPV感染情况呈正相关。
목적：비교형광정량취합매련반응법（간칭형광정량법）여기인심편분형법（간칭기인심편법）재검측인유두류병독（H PV ）중적민감도。방법선취2010년2월지2013년2월래해원부과문진취진적환자305례，수집기궁경탈락세포，분별용형광정량법화기인심편법검측 H PV ，대결과불일치적병례채용측서법진행학증。305례병례균진행액기세포학검측。결과형광정량법검측 HPV민감도위33．8％，기인심편법검측HPV민감도위37．6％，량충방법구유교고적일치성，HPV감염솔수궁경탈락세포이형성증가이증가。결론형광정량법화기인심편법검측 H PV구유교고적일치성，기중기인심편법민감도교고。궁경세포학개변적엄중정도여 HPV감염정황정정상관。
Objective To compare the sensitivity of the fluorescent quantitation PCR method (fluorescence quantitative method for short)and the gene chips typing method (gene chips method for short)in testing human papilloma virus(HPV) .Methods 305 outpatients in the gynecological clinic of the hospital from February 2010 to February 2013 were selected as the research subject . Then the cervical exfoliated cells were collected and detected HPV by the fluorescence quantitative method and the gene chips meth-od respectively .Those cases with inconsistent results were confirmed by the PCR sequencing method .305 cases were performed the liquid based cytology test .Results The sensitivity of the fluorescence quantitative method in detecting HPV was 33 .8% and which of the gene chips method was 37 .6% .The two methods had a higher conformance .The HPV infection rate was increased with the increase of cervical exfoliated cell atypia .Conclusion The fluorescence quantitative method and the gene chips method have a higher conformance .The sensitivity of the gene chips method is higher .The severity degree of cervical cytological change is positively cor-related with the HPV infection .