食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
3期
18-22
,共5页
小球藻%油脂提取%气相色谱
小毬藻%油脂提取%氣相色譜
소구조%유지제취%기상색보
chlorella%oil extracted%gas chromatography
小球藻因其高的油脂含量和生长特性成为生物柴油研究的重要微藻资源之一，而研究表明油脂提取方法的不同通常直接关系到生物柴油的质量。研究了索氏提取、有机溶剂浸提两种油脂提取方法及不同细胞前处理方法对小球藻油脂提取率的影响，并比较了不同方法提取的油脂组成成分差异。结果表明，在不同的提取方法中，油脂得率、油脂中中长链脂肪酸（C14-C22）占总脂肪酸比例及脂肪酸提取纯度各不相同。其中，氯仿-甲醇浸提法是一种简便快捷高效的油脂提取方法，其油脂得率为（22.40±0.89）%，中长链脂肪酸（C14-C22）比例为85.84%；索氏提取法中无水乙醇作为提取溶剂获得的得率最高为（24.37±0.21）%，石油醚提取的油脂脂肪酸成分最多，达到10种。
小毬藻因其高的油脂含量和生長特性成為生物柴油研究的重要微藻資源之一，而研究錶明油脂提取方法的不同通常直接關繫到生物柴油的質量。研究瞭索氏提取、有機溶劑浸提兩種油脂提取方法及不同細胞前處理方法對小毬藻油脂提取率的影響，併比較瞭不同方法提取的油脂組成成分差異。結果錶明，在不同的提取方法中，油脂得率、油脂中中長鏈脂肪痠（C14-C22）佔總脂肪痠比例及脂肪痠提取純度各不相同。其中，氯倣-甲醇浸提法是一種簡便快捷高效的油脂提取方法，其油脂得率為（22.40±0.89）%，中長鏈脂肪痠（C14-C22）比例為85.84%；索氏提取法中無水乙醇作為提取溶劑穫得的得率最高為（24.37±0.21）%，石油醚提取的油脂脂肪痠成分最多，達到10種。
소구조인기고적유지함량화생장특성성위생물시유연구적중요미조자원지일，이연구표명유지제취방법적불동통상직접관계도생물시유적질량。연구료색씨제취、유궤용제침제량충유지제취방법급불동세포전처리방법대소구조유지제취솔적영향，병비교료불동방법제취적유지조성성분차이。결과표명，재불동적제취방법중，유지득솔、유지중중장련지방산（C14-C22）점총지방산비례급지방산제취순도각불상동。기중，록방-갑순침제법시일충간편쾌첩고효적유지제취방법，기유지득솔위（22.40±0.89）%，중장련지방산（C14-C22）비례위85.84%；색씨제취법중무수을순작위제취용제획득적득솔최고위（24.37±0.21）%，석유미제취적유지지방산성분최다，체도10충。
Chlorella has become one of the important microalgae resources for biodiesel research because of its high fat content and growth characteristics,and there is a lot of research showing that the lipin extraction method is usually directly related to the quality of biodiesel.We investigate the Soxhlet extraction and organic solvent extraction of lipin extraction method and the influence of extraction rate of chlorella lipin extracted by different pre-cell approach methods, and compared the composition differences of lipin extracted by different methods.The results show that the different extraction methods result in different lipin yield ,different ratio of long-chain fatty acids (C14-C22)to total fatty acids and different extract purity of fatty acids.Among them,chloroform-methanol extraction method is a simple and efficient method of lipin extraction,with lipin yield of (22.40±0.89)%and 85.84%long chain fatty acids (C14-C22);In Soxhlet extraction,we get the highest yield rate of (24.37±0.21)%with Anhydrous alcohol as an extraction solvent,and we get up to 10 kinds of fatty acids with petroleum ether as an extraction solvent.
