食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
3期
1-3
,共3页
刘金娜%贾东升%马春英%刘铭%谢晓亮%杨太新%温春秀
劉金娜%賈東升%馬春英%劉銘%謝曉亮%楊太新%溫春秀
류금나%가동승%마춘영%류명%사효량%양태신%온춘수
蒲公英%黄酮%蛋白质%紫外分光光度法
蒲公英%黃酮%蛋白質%紫外分光光度法
포공영%황동%단백질%자외분광광도법
dandelion%lavonoids%protein%UV spectrophotometry
探讨不同类型蒲公英总黄酮及可溶性蛋白的动态变化规律。根据植物学特性及种质来源不同将蒲公英分为栽培种质1、栽培种质2及野生3种类型,采用紫外-可见分光光度法,分别在波长500 nm和595 nm下对蒲公英4个不同时期总黄酮和总蛋白的含量进行测定。结果表明:不同类型蒲公英中蛋白与黄酮含量随时间均呈现先增后减的趋势,180 d时含量均最高,其中栽培类型2中蛋白和黄酮的含量分别达(15.0547±0.6085) mg/g,(37.9840±0.7356)mg/g显著高于栽培类型1和野生类型。3种类型蒲公英黄酮与蛋白含量之间存在一定的正相关性。不同类型蒲公英中黄酮与蛋白的含量随时间变化显著。
探討不同類型蒲公英總黃酮及可溶性蛋白的動態變化規律。根據植物學特性及種質來源不同將蒲公英分為栽培種質1、栽培種質2及野生3種類型,採用紫外-可見分光光度法,分彆在波長500 nm和595 nm下對蒲公英4箇不同時期總黃酮和總蛋白的含量進行測定。結果錶明:不同類型蒲公英中蛋白與黃酮含量隨時間均呈現先增後減的趨勢,180 d時含量均最高,其中栽培類型2中蛋白和黃酮的含量分彆達(15.0547±0.6085) mg/g,(37.9840±0.7356)mg/g顯著高于栽培類型1和野生類型。3種類型蒲公英黃酮與蛋白含量之間存在一定的正相關性。不同類型蒲公英中黃酮與蛋白的含量隨時間變化顯著。
탐토불동류형포공영총황동급가용성단백적동태변화규률。근거식물학특성급충질래원불동장포공영분위재배충질1、재배충질2급야생3충류형,채용자외-가견분광광도법,분별재파장500 nm화595 nm하대포공영4개불동시기총황동화총단백적함량진행측정。결과표명:불동류형포공영중단백여황동함량수시간균정현선증후감적추세,180 d시함량균최고,기중재배류형2중단백화황동적함량분별체(15.0547±0.6085) mg/g,(37.9840±0.7356)mg/g현저고우재배류형1화야생류형。3충류형포공영황동여단백함량지간존재일정적정상관성。불동류형포공영중황동여단백적함량수시간변화현저。
Exploring the dynamic changes of total protein content and flavonoids content ,and analysing the correlation between protein and flavonoids. According to the botany characteristic and germplasm sources , dandelions were divided into three style denoted by cultivated germplasm 1,cultivated germplasm 2 and dandelion agriotype. Utilizing the method of ultraviolet-visible spectrophotometry , the content of total flavonoids was measured in the wavelength of 500 nm,and the content of total protein was measured in 595 nm. Results showed that both the content of total protein and total flavonoids in dandelion tended to increase then keeping a trend of decline,cultivated germplasm 2 approached the highest level in growth for 180 days and was higher than other types, there existed a positive correlation of protein and flavonoid in three types of dandelion. the contents of protein and flavonoid changed significantly in different times.