CAJ | 학술논문

目的：建立同时测定食品中9种水溶性维生素的高效液相色谱分析方法。方法采用C18色谱柱进行分离,以pH 2.5,25 mmol/L KH2PO4-乙腈二元体系为流动相进行梯度洗脱,最后用高效液相色谱二极管阵列检测器对9种维生素进行测定。结果在优化实验条件下,测得维生素C的线性范围为0.04~100μg/mL,硫胺、核黄素、烟酰胺和吡哆醇的线性范围为0.02~100μg/mL,泛酸的线性范围为0.08~400μg/mL,生物素的线性范围为0.08~200μg/mL,叶酸的线性范围为0.01~50μg/mL,氰钴胺的线性范围为0.04~100μg/mL；线性相关系数为0.9997~0.9999,相对标准偏差为0.28%~1.35%,检出限范围为3~45 ng/mL,加标回收率为90.6%~105.4%。结论该方法快速、准确、灵敏,适合测定营养强化食品中多种水溶性维生素。
목적：건립동시측정식품중9충수용성유생소적고효액상색보분석방법。방법채용C18색보주진행분리,이pH 2.5,25 mmol/L KH2PO4-을정이원체계위류동상진행제도세탈,최후용고효액상색보이겁관진렬검측기대9충유생소진행측정。결과재우화실험조건하,측득유생소C적선성범위위0.04~100μg/mL,류알、핵황소、연선알화필치순적선성범위위0.02~100μg/mL,범산적선성범위위0.08~400μg/mL,생물소적선성범위위0.08~200μg/mL,협산적선성범위위0.01~50μg/mL,청고알적선성범위위0.04~100μg/mL；선성상관계수위0.9997~0.9999,상대표준편차위0.28%~1.35%,검출한범위위3~45 ng/mL,가표회수솔위90.6%~105.4%。결론해방법쾌속、준학、령민,괄합측정영양강화식품중다충수용성유생소。
Objective To develop a high performance liquid chromatography (HPLC) method for the de-termination of nine water-soluble vitamins in foods. Methods The chromatographic separation was per-formed on a C18 column with gradient elution, using potassium dihydrogen phosphate and acetonitrile as mobile phase. The analyte was detected by HPLC with diode array detector. Results Under the optimal conditions, the calibration curves of nine vitamins were linear in the range of 0.04~100 μg/mL for vitamin C, 0.02~100μg/mL for thiamine, riboflavin, nicotinamide and pyridoxine;0.08~400μg/mL for pantothenic acid;0.08~200μg/mL for biotin; 0.01~50 μg/mL for folic acid; and 0.04~100 μg/mL for cyanocobalamin with correlation coefficient (r) more than 0.9997. The relative standard deviations (RSDs) were less than 1.35%, and the limit of detection (LOD) was found in the range of 3~45 ng/mL and the spiked recoveries were between 90.6~105.4%. The developed method was applied to determine the water-soluble vitamins in functional beverages, multivita-min tablets and fortified rice. Conclusion The proposed method is rapid, accurate and sensitive, and it is suitable for detecting water-soluble vitamins in fortified foods.