CAJ | 학술논문

随着越来越多的转基因作物获得商业化生产和种植，转基因农产品的安全问题一直都有异议，因此，无论对消费人群还是政府监管部门，快速检测转基因组分都是非常必要。转基因组分的检测通常以核酸为基础进行检测，而环介导等温扩增技术(LAMP)是一种等温条件下核酸扩增技术，具有高特异性、高灵敏度、操作简单、成本低廉、结果可视化及不需要专用设备的特点，已逐渐用于转基因组分的检测中。本文针对LAMP技术的原理、优点、关键技术及其在转基因大豆、玉米、水稻等农产品中的应用做一综述，从而为其更广泛的用于转基因组分的检测提供理论依据。
수착월래월다적전기인작물획득상업화생산화충식，전기인농산품적안전문제일직도유이의，인차，무론대소비인군환시정부감관부문，쾌속검측전기인조분도시비상필요。전기인조분적검측통상이핵산위기출진행검측，이배개도등온확증기술(LAMP)시일충등온조건하핵산확증기술，구유고특이성、고령민도、조작간단、성본저렴、결과가시화급불수요전용설비적특점，이축점용우전기인조분적검측중。본문침대LAMP기술적원리、우점、관건기술급기재전기인대두、옥미、수도등농산품중적응용주일종술，종이위기경엄범적용우전기인조분적검측제공이론의거。
As more and more genetically modified (GM) crops are approved for commercialization and planting, transgenic product security issues have been to the objection, therefore, regardless of the consumer or government regulators, the development of rapid detection method for genetically modified components is re-quired. Detection of genetically modified component are usually based on nucleic acid detection, and loop-mediated isothermal amplification (LAMP) is one isothermal nucleic acids amplification technique. It has the characteristics of high specificity, sensitivity, operator simple, low cost, visualization results and does not require special equipment. This technology has been increasingly used to detect genetically modified compo-nent. In this paper, we reviewed the LAMP technology principle, characteristics, key technology and its appli-cation in the main genetically modified soybeans, corn, rice etc, so it provides theoretical basis for its applica-tion in the detection of genetically modified component.