CAJ | 학술논문

目的：建立坚果中黄曲霉毒素 B1、B2、G1、G2的高效液相色谱荧光检测器测定方法。方法样品以甲醇-水(70：30, v/v)溶液匀质提取,过黄曲霉毒素总量免疫层析亲和柱净化,经LaChrom C18色谱柱分离和光化学柱后衍生反应器衍生后,用带有荧光检测器的高效液相色谱仪测定。采用峰面积外标法定量坚果中黄曲霉毒素B1、B2、G1、G2含量。结果四种黄曲霉毒素在各自的浓度范围内线性关系良好,相关系数均大于0.999, B1、B2、G1、G2的检出限依次为0.10、0.05、0.10、0.05μg/kg。在3个添加水平下回收率为77.5%~109.8%,相对标准偏差为1.43%~2.71%。结论该方法的灵敏度、准确度、精密度均符合黄曲霉毒素的检测技术要求,适用于坚果中黄曲霉毒素B1、B2、G1、G2的日常检测。
목적：건립견과중황곡매독소 B1、B2、G1、G2적고효액상색보형광검측기측정방법。방법양품이갑순-수(70：30, v/v)용액균질제취,과황곡매독소총량면역층석친화주정화,경LaChrom C18색보주분리화광화학주후연생반응기연생후,용대유형광검측기적고효액상색보의측정。채용봉면적외표법정량견과중황곡매독소B1、B2、G1、G2함량。결과사충황곡매독소재각자적농도범위내선성관계량호,상관계수균대우0.999, B1、B2、G1、G2적검출한의차위0.10、0.05、0.10、0.05μg/kg。재3개첨가수평하회수솔위77.5%~109.8%,상대표준편차위1.43%~2.71%。결론해방법적령민도、준학도、정밀도균부합황곡매독소적검측기술요구,괄용우견과중황곡매독소B1、B2、G1、G2적일상검측。
Objective To establish a high performance liquid chromatography (HPLC) coupled with post-column photochemical derivatization method for the determination of the aflatoxin B1, B2, G1, and G2 in nuts. Methods The sample was tested by HPLC with fluorescence detector after being extracted from metha-nol-water (70:30, v/v), purified by immunoaffinity chromatography column of aggregated aflatoxin, separated by LaChrom C18 chromatographic column, and derived from post column photochemical derivative reactor. The con-tents of aflatoxin B1, B2, G1, and G2 in nuts were measured using peak area external standard method. Results The calibration curves showed a good linearity in each concentration range with correlation coefficient greater than 0.999. The limits of detection (LODs) were 0.10, 0.05, 0.10, and 0.05μg/kg for the aflatoxins B1, B2, G1, and G2 in nuts, respectively. The recovery rate was within 77.5%~109.8%at three adding levels with the relative standard deviation (RSDs) of 1.43%~2.71%. Conclusion This method is sensitive, accurate and precise which is in accordance with technical standards of the aflatoxins detection. It is proper for routine detection of the aflatox-ins B1, B2, G1, and G2 in nuts.