CAJ | 학술논문

目的：综合评价4种不同厂家的黄曲霉毒素 B1试剂盒。方法选择几种食品基质(婴幼儿米粉、花生酱、酱油),分别用R-Biopharm, Romer, Helica和华安麦科的黄曲霉毒素B1试剂盒检测,将测定结果与液相色谱法测得结果进行比对。结果通过与液相色谱法测得结果比较,华安麦科试剂盒只适合检测婴幼儿米粉样品。Helica和R-Biopharm试剂盒能检测多种基质,而Helica试剂盒检测结果与液相相符度低, R-Biopharm试剂盒检测米粉时,本底较高。Romer试剂盒适用于花生酱和酱油样品。结论为了得到最佳的检测结果,针对不同的样品基质,应选择合适的试剂盒。当用酶联法检测出阳性样品时,需用液相色谱法确认。
목적：종합평개4충불동엄가적황곡매독소 B1시제합。방법선택궤충식품기질(영유인미분、화생장、장유),분별용R-Biopharm, Romer, Helica화화안맥과적황곡매독소B1시제합검측,장측정결과여액상색보법측득결과진행비대。결과통과여액상색보법측득결과비교,화안맥과시제합지괄합검측영유인미분양품。Helica화R-Biopharm시제합능검측다충기질,이Helica시제합검측결과여액상상부도저, R-Biopharm시제합검측미분시,본저교고。Romer시제합괄용우화생장화장유양품。결론위료득도최가적검측결과,침대불동적양품기질,응선택합괄적시제합。당용매련법검측출양성양품시,수용액상색보법학인。
Objective To compare and evaluate 4 kinds of aflatoxin B1 assay kits and liquid chromatography. Methods Three sample matrixes (infant formula rice flour, peanut butter and soy bean sauce) were detected using RIDASCREEN? aflatoxin B1 assay, AgraQuant? aflatoxin B1 assay, Helica low matrix aflatoxin B1 assay and Huaan magnech aflatoxin B1 assay, and then compared the analysis results with those by liquid chromatography. Results By contrast, Huaan magnech aflatoxin B1 assay applied to only the infant formula rice flour. HELICA low matrix aflatoxin B1 assay and RIDASCREEN? aflatoxin B1 assay could detect more sample matrixes, but the analysis results of Helica low matrix aflatoxin B1 assay were inconsistent with those by liquid chromatography, the background of RIDASCREEN? aflatoxin B1 assay was higher. AgraQuant? aflatoxin B1 assay were applied to peanut butter and soybean sauce. Conclusion In view of the different of the sample matrix, the suitable assay kit needed to be chosen to get the best analysis results. When the results of ELISA were positive, the HPLC method was needed for further validation.