CAJ | 학술논문

目的：针对茶叶中黄曲霉毒素 B1的检测，对比胶体金免疫层析法、高效液相色谱、酶联免疫法的差异。方法以红茶、绿茶、花茶为基质，进行方法验证。并选取具有代表性的红茶、绿茶、乌龙茶、及花茶、萃取茶、药用保健茶分别用不同的方法检测。结果改良后的液相法和胶体金免疫层析法准确度和精密度较高，酶联免疫法存在假阳性。随机检测11种茶叶仅有一种检出黄曲霉毒素B1。
목적：침대다협중황곡매독소 B1적검측，대비효체금면역층석법、고효액상색보、매련면역법적차이。방법이홍다、록다、화다위기질，진행방법험증。병선취구유대표성적홍다、록다、오룡다、급화다、췌취다、약용보건다분별용불동적방법검측。결과개량후적액상법화효체금면역층석법준학도화정밀도교고，매련면역법존재가양성。수궤검측11충다협부유일충검출황곡매독소B1。
Objectives To compare the three different methods for the determination of aflatoxin B1 in tea samples, including gold immune chromatography assay (GICA), high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). Methods Method validation was done based on black tea, green tea and jasmine tea. And black tea, green tea, oolong tea, jasmine tea, extracted tea, medicinal health tea as representatives were detected with different methods. Results Both of optimized GICA and HPLC achieved high accuracy and precision. ELISA assay produced fake positive. Only one of eleven tested samples had aflatoxin B1 in random test.