CAJ | 학술논문

目的：以鸡、猪等动物肝脏为研究对象，二氯甲烷为提取溶剂，建立免疫亲和柱净化、高效液相色谱-光化学衍生-荧光检测器检测六种黄曲霉毒素的分析方法。方法样品经提取、过滤、浓缩、复溶后经免疫亲和柱净化，进样后采用光化学衍生器在线对黄曲霉毒素 G1、B1衍生，荧光检测器分析 B1、B2、G1、G2、M1、M2六种黄曲霉毒素。结果在不同加标水平，不同肝脏回收率在70.2%~94.1%之间，变异系数为2.67%~12.90%，检测低限为0.16μg/kg。结论该方法的准确度与精密度较高，可以满足痕量分析要求。
목적：이계、저등동물간장위연구대상，이록갑완위제취용제，건립면역친화주정화、고효액상색보-광화학연생-형광검측기검측륙충황곡매독소적분석방법。방법양품경제취、과려、농축、복용후경면역친화주정화，진양후채용광화학연생기재선대황곡매독소 G1、B1연생，형광검측기분석 B1、B2、G1、G2、M1、M2륙충황곡매독소。결과재불동가표수평，불동간장회수솔재70.2%~94.1%지간，변이계수위2.67%~12.90%，검측저한위0.16μg/kg。결론해방법적준학도여정밀도교고，가이만족흔량분석요구。
Objective To develop a method for detecting six aflatoxins in chicken and pig liver. Methods Samples were extracted, filtered, concentrated and reconstituted purified by immunoaffinity column, derivatived aflatoxin G1, B1 using photochemical derivatization unit online after injection. Then six aflatoxins B1, B2, G1, G2, M1, and M2 were analyzed by fluorescence detector. Results The recoveries of samples with different spiking levels were 70.2%~94.1%. The RSD were 2.67%~12.90%. The lowest limit of quantification was 0.16 μg/kg. Conclusion This method is of high accuracy and precision, and can meet the requirements for trace analysis.