CAJ | 학술논문

目的：利用纳米金在盐诱导下凝聚后引起的颜色变化,建立基于非标记的 AFB1特异性结合适配体,针对AFB1的灵敏快速可视化检测方法。方法通过对适配体DNA浓度、NaCl盐溶液、pH值等条件的优化,确定最佳反应体系并进行特异性实验。结果本方法具有良好的灵敏度和特异性,线性范围和检测限分别是0.025～10 ng/mL和0.025 ng/mL,可以满足中国和欧盟的最大残留限量(MRL)的检测要求。结论该方法实现了对黄曲霉毒素B1的可视化检测,简单、高效、可行,具有较高的分析灵敏度和较好的特异性。
목적：이용납미금재염유도하응취후인기적안색변화,건립기우비표기적 AFB1특이성결합괄배체,침대AFB1적령민쾌속가시화검측방법。방법통과대괄배체DNA농도、NaCl염용액、pH치등조건적우화,학정최가반응체계병진행특이성실험。결과본방법구유량호적령민도화특이성,선성범위화검측한분별시0.025～10 ng/mL화0.025 ng/mL,가이만족중국화구맹적최대잔류한량(MRL)적검측요구。결론해방법실현료대황곡매독소B1적가시화검측,간단、고효、가행,구유교고적분석령민도화교호적특이성。
Objective The colorimetric detection of AFB1 based on a label-free the AFB1 aptasensor using unmodified gold nanoparticles(AuNPs) was developed in the present study. Methods Concentration of DNA and NaCl was optimized, as well as the pH values , to determine the optimal reaction system. Specific experi-ment was carried out with the interference of OTA. Results The linear dynamic range and detection sensitiv-ity were found to be 0.025-10 ng/mL and 0.025 ng/mL of AFB1 respectively, which were lower than the maxi-mum residue limit (MRL) in edible food, as set by China and the European Commission. Conclusion This study provides a simple, sensitive and visible detection method for AFB1 analysis, which can be applied in fu-ture on-site detection for food and agricultural products.