CAJ | 학술논문

目的：基于拉曼光谱技术初步探讨菠菜表面有机磷农药毒死蜱的快速、无损检测新方法。方法利用本研究自行搭建的拉曼光谱检测系统获取残留不同浓度毒死蜱农药的菠菜光谱曲线,通过SG-5点平滑减小曲线噪声,基于最小二乘法的10次多项式拟合法剔除菠菜荧光背景,并对含有不同浓度毒死蜱农药的菠菜样品拉曼图谱进行比较分析。结果特征归属为P=S振动的632 cm-1处的拉曼信号可识别菠菜表面毒死蜱农药残留,其检测限为400 mg/kg,而且拉曼特征波峰的相对强度与菠菜表面农药残留浓度存在较好的线性关系,其相关系数为R2=0.92。结论拉曼光谱技术有望实现叶片类蔬菜的农药残留的快速、无损、定量检测。
목적：기우랍만광보기술초보탐토파채표면유궤린농약독사비적쾌속、무손검측신방법。방법이용본연구자행탑건적랍만광보검측계통획취잔류불동농도독사비농약적파채광보곡선,통과SG-5점평활감소곡선조성,기우최소이승법적10차다항식의합법척제파채형광배경,병대함유불동농도독사비농약적파채양품랍만도보진행비교분석。결과특정귀속위P=S진동적632 cm-1처적랍만신호가식별파채표면독사비농약잔류,기검측한위400 mg/kg,이차랍만특정파봉적상대강도여파채표면농약잔류농도존재교호적선성관계,기상관계수위R2=0.92。결론랍만광보기술유망실현협편류소채적농약잔류적쾌속、무손、정량검측。
Objective To explore a rapid and nondestructive method for the determination of pesticide re-sidue on the surface of spinach by Raman spectroscopy. Methods Raman spectral signals from different con-centrations of pesticide on spinach were acquired by laboratory-setting-up Raman spectroscopy detection sys-tem. Savitzky-Golay five-point filter was used to smooth the original Raman spectra, the 10th order polynomial fitting based on the partial least square was used to remove the fluorescence background from Raman signals, and compared the Raman spectrum of spinach containing different concentration chlorpyrifos pesticide. Results Chlorpyrifos pesticide residue on spinach surface could be detected based on 632 cm-1 Raman signal (chemical bonds vibration of P=S), the minimum value of chlorpyrifos pesticide on spinach surface was 400 mg/kg, and there was a good linear correlation between the concentrations of pesticide residue on spinach’s surface and the relative intensity of Raman signals. The correlation coefficient of this study was 0.92. Conclusion The study demonstrated that Raman spectroscopy had a great potential for rapid and non-destructive detection of pesticide residue on spinach’s surface.