CAJ | 학술논문

目的：为实现水中四环素残留的快速检测,探索建立四环素水溶液的表面增强拉曼光谱(surface en-hanced Raman spectroscopy, SERS)检测方法。方法以OTR202和OTR103作为表面增强基底,分析四环素水溶液的SERS光谱,应用自适应迭代惩罚最小二乘法(air-PLS)扣除SERS的荧光背景,探讨样品加入量对SERS信号强度的影响,以1274 cm-1作为特征峰建立四环素水溶液的标准曲线。结果确定了四环素水溶液的加入量为20μL,建立的线性回归方程Y=257.47X+85.165,相关系数r为0.9897。结论本研究方法无需前处理,简便快捷,为后续水中四环素残留快速现场检测奠定了良好基础。
목적：위실현수중사배소잔류적쾌속검측,탐색건립사배소수용액적표면증강랍만광보(surface en-hanced Raman spectroscopy, SERS)검측방법。방법이OTR202화OTR103작위표면증강기저,분석사배소수용액적SERS광보,응용자괄응질대징벌최소이승법(air-PLS)구제SERS적형광배경,탐토양품가입량대SERS신호강도적영향,이1274 cm-1작위특정봉건립사배소수용액적표준곡선。결과학정료사배소수용액적가입량위20μL,건립적선성회귀방정Y=257.47X+85.165,상관계수r위0.9897。결론본연구방법무수전처리,간편쾌첩,위후속수중사배소잔류쾌속현장검측전정료량호기출。
Objective To establish a method for rapid detection of tetracycline residual in water by sur-face enhanced Raman spectroscopy (SERS). Methods OTR202 and OTR103 were used as enhanced substrate of SERS, and the fluorescence backgrounds of SERS were subtracted by using adaptive iteratively reweighted penalized least squares(air-PLS). The effect of the addition amount of tetracycline solution on SERS intensity was discussed, and 1374 cm-1 was used as the characteristic peak to establish the standard curve of tetracycline solution. Results 20 μL was determined as the best addition amount, the linear regression equation was Y=257.47X+85.165, and the correlation coefficient r was 0.9897. Conclusion A fast and simple method without pretreatment was proposed to detect tetracycline solution, and it provided a reference for the following rapid and field detection of tetracycline residual in water.