茶叶通讯
茶葉通訊
다협통신
TEA COMMUNICATION
2013年
4期
6-9,28
,共5页
黄安平%李正文%肖蕾%王沅江%周凌云
黃安平%李正文%肖蕾%王沅江%週凌雲
황안평%리정문%초뢰%왕원강%주릉운
茶树%茶刺蛾%棒须刺蛾寄蝇%挥发性有机化合物%虫害诱导植物
茶樹%茶刺蛾%棒鬚刺蛾寄蠅%揮髮性有機化閤物%蟲害誘導植物
다수%다자아%봉수자아기승%휘발성유궤화합물%충해유도식물
Tea [Camwllia sinensis (Ericales:Theaceae)]%Tea slug moth (Iragoides fasciata Moore)%Chaetexorista palpis chao%Volatile Organic Compounds(VOCs)%Herbivore-Indrced Plant Volatiles(HIPV)
茶园观察发现,茶刺蛾危害的茶园的棒须刺蛾寄蝇数量明显多于未危害的茶园。推测茶树被茶刺蛾危害后释放了特定的信号物质,这些物质在棒须刺蛾寄蝇寄主定位中起着关键的作用。为了证实这一猜想,我们研究了茶刺蛾危害对茶树挥发性有机物释放的影响。未受茶刺蛾危害的枝叶有挥发性有机物74种组分。与未受茶刺蛾危害枝叶的挥发物组分相比较,受害后酯类、醛类和烃类的种类数量和所占相对含量都有显著变化。醇、杂环化合物、醚和有机酸类化合物在种类数量和相对含量上变化不大。受害后,4-异丙基甲苯、蒎烯、1-乙基-2,4-二甲基苯、4-乙基甲苯、3-乙基-甲苯、6-甲基庚烯[5]酮[2]和1,2,4-三甲基苯的相对含量显著增加。新产生54种化合物,其中烯烃14种,芳香烃12种,酯8种,烷烃8种,酮5种,醇3种,醛2种,有机酸和杂环类各1种。值得注意的是受害后萜类化合物的种类数量和相对含量均有显著的变化,暗示这些化合物可能在棒须刺蛾寄蝇的寄主寻找过程中发挥作用。研究结果为筛选引诱天敌昆虫的化学信息素,并应用其防控茶刺蛾的研究奠定一定的基础。
茶園觀察髮現,茶刺蛾危害的茶園的棒鬚刺蛾寄蠅數量明顯多于未危害的茶園。推測茶樹被茶刺蛾危害後釋放瞭特定的信號物質,這些物質在棒鬚刺蛾寄蠅寄主定位中起著關鍵的作用。為瞭證實這一猜想,我們研究瞭茶刺蛾危害對茶樹揮髮性有機物釋放的影響。未受茶刺蛾危害的枝葉有揮髮性有機物74種組分。與未受茶刺蛾危害枝葉的揮髮物組分相比較,受害後酯類、醛類和烴類的種類數量和所佔相對含量都有顯著變化。醇、雜環化閤物、醚和有機痠類化閤物在種類數量和相對含量上變化不大。受害後,4-異丙基甲苯、蒎烯、1-乙基-2,4-二甲基苯、4-乙基甲苯、3-乙基-甲苯、6-甲基庚烯[5]酮[2]和1,2,4-三甲基苯的相對含量顯著增加。新產生54種化閤物,其中烯烴14種,芳香烴12種,酯8種,烷烴8種,酮5種,醇3種,醛2種,有機痠和雜環類各1種。值得註意的是受害後萜類化閤物的種類數量和相對含量均有顯著的變化,暗示這些化閤物可能在棒鬚刺蛾寄蠅的寄主尋找過程中髮揮作用。研究結果為篩選引誘天敵昆蟲的化學信息素,併應用其防控茶刺蛾的研究奠定一定的基礎。
다완관찰발현,다자아위해적다완적봉수자아기승수량명현다우미위해적다완。추측다수피다자아위해후석방료특정적신호물질,저사물질재봉수자아기승기주정위중기착관건적작용。위료증실저일시상,아문연구료다자아위해대다수휘발성유궤물석방적영향。미수다자아위해적지협유휘발성유궤물74충조분。여미수다자아위해지협적휘발물조분상비교,수해후지류、철류화경류적충류수량화소점상대함량도유현저변화。순、잡배화합물、미화유궤산류화합물재충류수량화상대함량상변화불대。수해후,4-이병기갑분、파희、1-을기-2,4-이갑기분、4-을기갑분、3-을기-갑분、6-갑기경희[5]동[2]화1,2,4-삼갑기분적상대함량현저증가。신산생54충화합물,기중희경14충,방향경12충,지8충,완경8충,동5충,순3충,철2충,유궤산화잡배류각1충。치득주의적시수해후첩류화합물적충류수량화상대함량균유현저적변화,암시저사화합물가능재봉수자아기승적기주심조과정중발휘작용。연구결과위사선인유천활곤충적화학신식소,병응용기방공다자아적연구전정일정적기출。
Observed in the tea garden, we found that the number of Chaetexorista palpis Chao in the tea plantations where tea plants were damaged by tea slug moth was significantly more than those undamaged. We speculate that tea plant would release specific signal substances which play a key role in host location(especially long distance)by C. palpis. In order to confirm this conjecture, the effect of the damaging of tea slug moth on the volatile organic compounds (VOCs)of tea plant was studied. The results indicated that a total of seventy-four components were identified in the undamaged twigs and leaves. Compared with the undamaged twigs and leaves, the number and relative content of ethers, aldehydes, and hydrocarbons changed evidently, while those of alcohols, heterocyclic compounds, ethers and organic acid compounds had little change. After damage, the relative content of 4-cymene, pinene, 1-ethyl-2,4-dimethylphenoxy, 4-ethyltoluene, toluene-ethyl- 6-methyl- heptene [5]ketone [2] and 1,2,4- trimethylbenzene increased significantly. fifty-four new compounds were founded in the damaged ones, including of fourteen alkenes, twelve aromatic hydrocarbons, eight esters, eight alkanes, five ketones, three alcohols, two aldehydes, one heterocyclic and one organic acid. It is worth noting that the number and relative content of terpenoids significantly changed, suggesting that these compounds maybe play an important role on the host finding by C. palpis.The results can lay a foundation for screening infochemicals which can be used as a lure for natural enemies of insects and applied to control I. fasciata Moore.