湖南师范大学学报（医学版）
湖南師範大學學報（醫學版）
호남사범대학학보（의학판）
JOURNAL OF HUNAN NORMAL UNIVERSITY(MEDICAL SCIENCE)
2013年
3期
4-7
,共4页
田朗%朱咏贵%成亮%杨作成
田朗%硃詠貴%成亮%楊作成
전랑%주영귀%성량%양작성
柯萨奇病毒B3%细胞凋亡%Hela细胞
柯薩奇病毒B3%細胞凋亡%Hela細胞
가살기병독B3%세포조망%Hela세포
viral myocarditis%apoptosis%Hela cell
目的：观察CVB3感染对Hela细胞形态及凋亡变化的影响。方法：以CVB3感染Hela细胞建立病毒性感染细胞模型，观察细胞形态学变化，并以FITC-Annexin V联合PI双染色定量检测凋亡细胞。结果：CVB3感染Hela细胞后，可引起细胞病变效应；CVB3感染Hela细胞后，在一定时间段内凋亡程度随时间延长而增高。病毒组各时间点Hela细胞凋亡率明显高于对照组(P<0.05)。结论：CVB3感染Hela细胞后可使Hela细胞发生凋亡，并随着病毒感染时间的延长，细胞凋亡增多。
目的：觀察CVB3感染對Hela細胞形態及凋亡變化的影響。方法：以CVB3感染Hela細胞建立病毒性感染細胞模型，觀察細胞形態學變化，併以FITC-Annexin V聯閤PI雙染色定量檢測凋亡細胞。結果：CVB3感染Hela細胞後，可引起細胞病變效應；CVB3感染Hela細胞後，在一定時間段內凋亡程度隨時間延長而增高。病毒組各時間點Hela細胞凋亡率明顯高于對照組(P<0.05)。結論：CVB3感染Hela細胞後可使Hela細胞髮生凋亡，併隨著病毒感染時間的延長，細胞凋亡增多。
목적：관찰CVB3감염대Hela세포형태급조망변화적영향。방법：이CVB3감염Hela세포건립병독성감염세포모형，관찰세포형태학변화，병이FITC-Annexin V연합PI쌍염색정량검측조망세포。결과：CVB3감염Hela세포후，가인기세포병변효응；CVB3감염Hela세포후，재일정시간단내조망정도수시간연장이증고。병독조각시간점Hela세포조망솔명현고우대조조(P<0.05)。결론：CVB3감염Hela세포후가사Hela세포발생조망，병수착병독감염시간적연장，세포조망증다。
Objective To observe the changes of morphology and apoptosis in Hela cell infected with CVB3. Methods CVB3 were employed to infect the Hela cells to establish viral infection model. The changes of cell morphology were observed, and the apoptosis of Hela cells were measured after dyed with FITC-Annexin V/PI. Results After infected with CVB3, the cytopathic effect of the Hela cells was observed. And the apoptosis of Hela cell increased with time. The apoptosis rate in virus groups were significantly higher than that in control groups (P< 0.05). Conclusion Apoptosis were found in Hela cells infected with CVB3 and the apoptotic cells increased with time extension.
