发育医学电子杂志
髮育醫學電子雜誌
발육의학전자잡지
Journal of Developmental Medicine (Electronic Version)
2013年
4期
205-209
,共5页
长链非编码RNA%PVT1%乳腺癌
長鏈非編碼RNA%PVT1%乳腺癌
장련비편마RNA%PVT1%유선암
long noncoding RNA%PVT1%Breast cancer
目的：探讨长链非编码RNA PVT1（lncRNA-PVT1）对于乳腺癌细胞系MDA-MB-231增殖和侵袭的影响。方法构建pcDNA3.1-PVT1表达载体，并将其转染入MDA-MB-231细胞，用实时定量PCR方法检测转染细胞中lncRNA-PVT1的表达，用Edu标记技术检测转染细胞的增殖能力，用Transwell实验检测转染细胞的侵袭能力。结果构建成功的pcDNA3.1-PVT1重组质粒转染入MDA-MB-231细胞后lncRNA-PVT1表达增加；转染细胞的增殖能力增强；转染细胞的侵袭能力增强。结论lncRNA-PVT1能增强MDA-MB-231细胞的增殖和侵袭能力。
目的：探討長鏈非編碼RNA PVT1（lncRNA-PVT1）對于乳腺癌細胞繫MDA-MB-231增殖和侵襲的影響。方法構建pcDNA3.1-PVT1錶達載體，併將其轉染入MDA-MB-231細胞，用實時定量PCR方法檢測轉染細胞中lncRNA-PVT1的錶達，用Edu標記技術檢測轉染細胞的增殖能力，用Transwell實驗檢測轉染細胞的侵襲能力。結果構建成功的pcDNA3.1-PVT1重組質粒轉染入MDA-MB-231細胞後lncRNA-PVT1錶達增加；轉染細胞的增殖能力增彊；轉染細胞的侵襲能力增彊。結論lncRNA-PVT1能增彊MDA-MB-231細胞的增殖和侵襲能力。
목적：탐토장련비편마RNA PVT1（lncRNA-PVT1）대우유선암세포계MDA-MB-231증식화침습적영향。방법구건pcDNA3.1-PVT1표체재체，병장기전염입MDA-MB-231세포，용실시정량PCR방법검측전염세포중lncRNA-PVT1적표체，용Edu표기기술검측전염세포적증식능력，용Transwell실험검측전염세포적침습능력。결과구건성공적pcDNA3.1-PVT1중조질립전염입MDA-MB-231세포후lncRNA-PVT1표체증가；전염세포적증식능력증강；전염세포적침습능력증강。결론lncRNA-PVT1능증강MDA-MB-231세포적증식화침습능력。
Objectives To study the effect of ectopic expression of long Noncoding RNA PVT1 in MDA-MB-231 cells. Methods The pcDNA3.1-PVT1 plasmids were transfected into MDA-MB-231 cells and the expression of lncRNA-PVT1 was detected by Real time PCR. The proliferation ability of transfected cells was evaluated by Edu based assay. The invasion ability of transfected cells was evaluated by Transwell experiment. Results The expression of lncRNA-PVT1 was increased when the recombinant plasmids were transfected into MDA-MB-231 cells. The proliferation ability of transfected cells was improved and so was the invasion ability. Conclusion Long noncoding RNA PVT1 can improve the proliferation and invasion of MDA-MB-231 cells.
