CAJ | 학술논문

当外界生存环境发生变化时，变异链球菌可在短时间内通过诱导或抑制某些蛋白质表达而改变自身蛋白质的表达谱，使其能够在极端环境中存活。在pH5.5和有氧环境下，变异链球菌的蛋白质表达变化迅速且复杂，或上调或下降。细菌胞外的高渗透压可使变异链球菌通过激活或灭活特异性的酶和转运蛋白以及改变基因表达谱来调整其生理机能，在高渗透压环境下培养，变异链球菌的蛋白质点或上调或下降。充足的营养对变异链球菌的生长非常重要，为适应营养缺乏的生存环境，变异链球菌的细胞大小和脂肪酸组成会发生改变，其总的蛋白质合成率也会下降。在环境温度变化的应激状态下，变异链球菌细胞内会产生变性或异常的蛋白质，所产生的热休克蛋白可使蛋白质肽链重新折叠，恢复蛋白质原来的构象及原有的功能，其蛋白质或上调或下调。在某些中草药提取物存在的情况下，变异链球菌的蛋白质表达明显下调。应用蛋白组学技术，可以发现在不同应激状态下变异链球菌的蛋白质表达图谱的改变，这可以揭示变异链球菌致龋的机制，从而为龋病的防治提供科学依据。
당외계생존배경발생변화시，변이련구균가재단시간내통과유도혹억제모사단백질표체이개변자신단백질적표체보，사기능구재겁단배경중존활。재pH5.5화유양배경하，변이련구균적단백질표체변화신속차복잡，혹상조혹하강。세균포외적고삼투압가사변이련구균통과격활혹멸활특이성적매화전운단백이급개변기인표체보래조정기생리궤능，재고삼투압배경하배양，변이련구균적단백질점혹상조혹하강。충족적영양대변이련구균적생장비상중요，위괄응영양결핍적생존배경，변이련구균적세포대소화지방산조성회발생개변，기총적단백질합성솔야회하강。재배경온도변화적응격상태하，변이련구균세포내회산생변성혹이상적단백질，소산생적열휴극단백가사단백질태련중신절첩，회복단백질원래적구상급원유적공능，기단백질혹상조혹하조。재모사중초약제취물존재적정황하，변이련구균적단백질표체명현하조。응용단백조학기술，가이발현재불동응격상태하변이련구균적단백질표체도보적개변，저가이게시변이련구균치우적궤제，종이위우병적방치제공과학의거。
When external environment changes, Streptococcus mutans(S.mutans) can change its protein expression profile through induction or inhibition of some protein expressions to ensure survival in extreme environments. Under pH 5.5 and aerobic condition, the protein expression of S.mutans changes rapidly in a complicated manner by increasing or decreasing. The extracellular high osmotic pressure of bacteria can induce S.mutans to adjust its physiological function by activating or inactivating some special enzymes and transport proteins and changing the gene expression profile. When cultivated under high osmotic pressure, protein points of S.mutans may be increased or reduced. Adequate nutrition is very important for the growth of S.mutans. To adapt to lack of nutrition, cell size and composition of S.mutans fatty acid change with decrease in total synthesis rate of proteins. When environment temperature changes, the cells will produce denatured or abnormal proteins with increased or reduced expressions. The heat shock protein produced can induce protein peptides to refold; it can also restore the original conformation and function of proteins. Some Chinese herbal medicine extracts can significantly reduce the protein expression of S.mutans. With the application of proteomics techniques, changes in S.mutans protein expression pattern can be found under different stress states. These techniques reveal information on the mechanism of S.mutans to caries and provide a scientific basis for prevention and control of caries.