中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
24期
11503-11506
,共4页
李忠军%邓跃飞%庞家栋%张黎明
李忠軍%鄧躍飛%龐傢棟%張黎明
리충군%산약비%방가동%장려명
神经胶质瘤%转铁蛋白%氧化石墨烯%光热治疗
神經膠質瘤%轉鐵蛋白%氧化石墨烯%光熱治療
신경효질류%전철단백%양화석묵희%광열치료
Glioma%Transferrin%Graphene oxide%Photothermy
目的：观察在近红外线（near infrared，NIR）激光照射下功能化纳米氧化石墨烯（NGO-Tf-FITC ）微粒对脑胶质瘤 U251细胞的靶向光热作用。方法将已成功制备的单层纳米氧化石墨烯（nano-graphene oxide，NGO）偶联靶向分子转铁蛋白（transferrin，Tf）及荧光分子异硫氰酸荧光素（fluorescein isothiocyanate，FITC）的功能化NGO-Tf-FITC微粒，与U251脑胶质瘤细胞孵育培养，通过CCK8细胞活力检测判定其细胞毒性，并在808 nm NIR 照射后流式细胞仪检测对细胞杀伤效果。结果按0.1、1.0、3.0和5.0 mg/ml浓度NGO-Tf-FITC分4组与U251细胞孵育，48 h后酶标仪测吸光度值分别为0.747±0.031、0.732±0.043、0.698±0.051和0.682±0.039，空白组为0.759±0.052，各组与空白组比较差异均无统计学意义（P＞0.05）；NGO-Tf-FITC组、NGO-FITC组和空白对照组细胞凋亡和死亡指数分别为（73.6±3.41）%、（52.6±2.66）%和（51.2±2.93）%，NGO-Tf-FITC组分别与NGO-FITC组和空白组比较差异均有统计学意义（P＜0.05），NGO-FITC组与空白组相比较差异无统计学意义（P＞0.05）。结论功能化NGO-Tf-FITC微粒细胞毒性低，且对脑胶质瘤U251细胞具有显著靶向光热杀灭作用。
目的：觀察在近紅外線（near infrared，NIR）激光照射下功能化納米氧化石墨烯（NGO-Tf-FITC ）微粒對腦膠質瘤 U251細胞的靶嚮光熱作用。方法將已成功製備的單層納米氧化石墨烯（nano-graphene oxide，NGO）偶聯靶嚮分子轉鐵蛋白（transferrin，Tf）及熒光分子異硫氰痠熒光素（fluorescein isothiocyanate，FITC）的功能化NGO-Tf-FITC微粒，與U251腦膠質瘤細胞孵育培養，通過CCK8細胞活力檢測判定其細胞毒性，併在808 nm NIR 照射後流式細胞儀檢測對細胞殺傷效果。結果按0.1、1.0、3.0和5.0 mg/ml濃度NGO-Tf-FITC分4組與U251細胞孵育，48 h後酶標儀測吸光度值分彆為0.747±0.031、0.732±0.043、0.698±0.051和0.682±0.039，空白組為0.759±0.052，各組與空白組比較差異均無統計學意義（P＞0.05）；NGO-Tf-FITC組、NGO-FITC組和空白對照組細胞凋亡和死亡指數分彆為（73.6±3.41）%、（52.6±2.66）%和（51.2±2.93）%，NGO-Tf-FITC組分彆與NGO-FITC組和空白組比較差異均有統計學意義（P＜0.05），NGO-FITC組與空白組相比較差異無統計學意義（P＞0.05）。結論功能化NGO-Tf-FITC微粒細胞毒性低，且對腦膠質瘤U251細胞具有顯著靶嚮光熱殺滅作用。
목적：관찰재근홍외선（near infrared，NIR）격광조사하공능화납미양화석묵희（NGO-Tf-FITC ）미립대뇌효질류 U251세포적파향광열작용。방법장이성공제비적단층납미양화석묵희（nano-graphene oxide，NGO）우련파향분자전철단백（transferrin，Tf）급형광분자이류청산형광소（fluorescein isothiocyanate，FITC）적공능화NGO-Tf-FITC미립，여U251뇌효질류세포부육배양，통과CCK8세포활력검측판정기세포독성，병재808 nm NIR 조사후류식세포의검측대세포살상효과。결과안0.1、1.0、3.0화5.0 mg/ml농도NGO-Tf-FITC분4조여U251세포부육，48 h후매표의측흡광도치분별위0.747±0.031、0.732±0.043、0.698±0.051화0.682±0.039，공백조위0.759±0.052，각조여공백조비교차이균무통계학의의（P＞0.05）；NGO-Tf-FITC조、NGO-FITC조화공백대조조세포조망화사망지수분별위（73.6±3.41）%、（52.6±2.66）%화（51.2±2.93）%，NGO-Tf-FITC조분별여NGO-FITC조화공백조비교차이균유통계학의의（P＜0.05），NGO-FITC조여공백조상비교차이무통계학의의（P＞0.05）。결론공능화NGO-Tf-FITC미립세포독성저，차대뇌효질류U251세포구유현저파향광열살멸작용。
Objective To observe the photothermy of functionalized nano-graphene oxide (NGO) particles in the near infrared (NIR) laser irradiation on brain glioma U251 Cells. Methods Single-layer nano-GO particles connected with transferrin (Tf) and fluorescein isothiocyanate (FITC) through conjugation to preparation functionalized NGO-Tf-FITC particles. After incubated with glioma U251 cells, determined its cytotoxicity with CCK8 cell viability testing and the cell kill effection with flow cytometery (FCM) after 808-nm NIR irradiation. Results According to 0.1, 1.0, 3.0 and 5.0 mg/ml concentration, NGO-Tf-FITC of four groups incubated with brain glioma U251 Cells. After 48 hours, ELIASA measuring absorbance values were 0.747±0.031,0.732± 0.043,0.698±0.051 and 0.682±0.039, blank group was 0.759±0.052, there were not statistic differences on every groups compared with blank groups(P>0.05);NGO-Tf-FITC group, NGO-FITC group and blank group, apoptosis and death index respectively(73.6 ± 3.41)%, (52.6 ± 2.66)% and (51.2 ± 2.93)%. Compared with blank group, the differences were statistically significant in NGO-Tf-FITC group and NGO-FITC group(P<0.05), but there was not statistical y significant difference in NGO-FITC group compared with blank group(P>0.05). Conclusion Functionalized NGO-Tf-FITC particles have low cytotoxicity and remarkable targeted photothermy on glioma U251 cells.
