中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
24期
11473-11475
,共3页
糖尿病,2型%内皮细胞%胰高血糖素样肽1%细胞增殖%内皮生长因子
糖尿病,2型%內皮細胞%胰高血糖素樣肽1%細胞增殖%內皮生長因子
당뇨병,2형%내피세포%이고혈당소양태1%세포증식%내피생장인자
Diabetes mellitus,type 2%Endothelial cells%Glucagon-like peptide 1%Cell proliferation%Endothelial growth factors
目的：探讨胰高糖素样肽1（GLP-1）对高糖培养的人脐静脉内皮细胞增殖的影响及其可能机制。方法人脐静脉内皮细胞分组培养，正常葡萄糖组（NG，葡萄糖浓度5.5 mmol/L），高葡萄糖组（HG，葡萄糖浓度33 mmol/L），高糖加GLP-1干预组（分为低剂量组，中剂量和高剂量组，浓度分别为1、10、20 nmol/L，分别记为C1、C2、C3组），于培养24 h，48 h和72 h用MTT法检测细胞增殖情况，并用ELISA法检测培养基上清液中血管内皮细胞生长因子（vascular endothelial growth factor，VEGF)的浓度。结果48 h后高糖组细胞增殖明显低于正常对照组，GLP-1干预后能明显促进细胞增殖，以中剂量最为明显（0.6±0.11 vs.0.47±0.09，P＜0.05），72 h这种情况更为明显；高糖培养48 h细胞分泌VEGF明显减少[（101.72±6.82） ng/ml vs.（184.66±14.06 ng/ml），P＜0.05]，GLP-1干预后VEGF水平明显增加（P＜0.05），尤其是中剂量组，72 h结果相似。结论 GLP-1能促进细胞分泌VEGF从而改善高糖导致的内皮细胞增殖能力下降。
目的：探討胰高糖素樣肽1（GLP-1）對高糖培養的人臍靜脈內皮細胞增殖的影響及其可能機製。方法人臍靜脈內皮細胞分組培養，正常葡萄糖組（NG，葡萄糖濃度5.5 mmol/L），高葡萄糖組（HG，葡萄糖濃度33 mmol/L），高糖加GLP-1榦預組（分為低劑量組，中劑量和高劑量組，濃度分彆為1、10、20 nmol/L，分彆記為C1、C2、C3組），于培養24 h，48 h和72 h用MTT法檢測細胞增殖情況，併用ELISA法檢測培養基上清液中血管內皮細胞生長因子（vascular endothelial growth factor，VEGF)的濃度。結果48 h後高糖組細胞增殖明顯低于正常對照組，GLP-1榦預後能明顯促進細胞增殖，以中劑量最為明顯（0.6±0.11 vs.0.47±0.09，P＜0.05），72 h這種情況更為明顯；高糖培養48 h細胞分泌VEGF明顯減少[（101.72±6.82） ng/ml vs.（184.66±14.06 ng/ml），P＜0.05]，GLP-1榦預後VEGF水平明顯增加（P＜0.05），尤其是中劑量組，72 h結果相似。結論 GLP-1能促進細胞分泌VEGF從而改善高糖導緻的內皮細胞增殖能力下降。
목적：탐토이고당소양태1（GLP-1）대고당배양적인제정맥내피세포증식적영향급기가능궤제。방법인제정맥내피세포분조배양，정상포도당조（NG，포도당농도5.5 mmol/L），고포도당조（HG，포도당농도33 mmol/L），고당가GLP-1간예조（분위저제량조，중제량화고제량조，농도분별위1、10、20 nmol/L，분별기위C1、C2、C3조），우배양24 h，48 h화72 h용MTT법검측세포증식정황，병용ELISA법검측배양기상청액중혈관내피세포생장인자（vascular endothelial growth factor，VEGF)적농도。결과48 h후고당조세포증식명현저우정상대조조，GLP-1간예후능명현촉진세포증식，이중제량최위명현（0.6±0.11 vs.0.47±0.09，P＜0.05），72 h저충정황경위명현；고당배양48 h세포분비VEGF명현감소[（101.72±6.82） ng/ml vs.（184.66±14.06 ng/ml），P＜0.05]，GLP-1간예후VEGF수평명현증가（P＜0.05），우기시중제량조，72 h결과상사。결론 GLP-1능촉진세포분비VEGF종이개선고당도치적내피세포증식능력하강。
Objective To study the effect and possible mechanism of Glucagon-like peptide-1 (GLP-1) for human umbilical vein endothelial cells (HUVECs) generation in high glucose nutrient medium. Methods HUVECs cultured in groups: normal glucose group(NG, glucose level was 5.5 mmol/L), high glucose group(HG, glucose level was 33 mmol/L), high glucose combined with GLP-1groups(low dose group, medium dose group and high dose group, GLP-1 level were 1 nmol/L, 10 nmol/L and 20 nmol/L respectively and marked group C1, group C2 and group C3), cell multiplication of groups were detected at 24 hours, 48 hours and 72 hours via MTT method and VEGF levels of supernatant were detected with ELISA kit at the same time. Results The cell multiplication of high glucose group was significantly declined than that of the normal group and increased by GLP-1, especially medium dose group (0.6±0.11 vs. 0.47±0.09, P<0.05). This phenomenon was more notable at 72 hours. When cells were cultured with high glucose medium for 48 hours VEGF level was significantly decreased than the control group, (101.72±6.82)ng/ml vs. (184.66±14.06)ng/ml, P<0.05, and increased significantly by GLP-1(P<0.05), the medium dose group was more notable, and the results of 72 hours were similar to the 48 hours.Conclusion GLP-1 can improve the multiplication of endothelial cells declined by high glucose through promoting VEGF secretion.
